Department of Biochemistry, Faculty of Science, Ain Shams University, Cairo, Egypt.
Nutrition. 2013 Jan;29(1):258-67. doi: 10.1016/j.nut.2012.06.004. Epub 2012 Oct 22.
The identification of the active phenolic compounds in the mixed extract of sea cucumber (Holothuria atra) body wall by high-performance liquid chromatography and an assessment of its hepatoprotective activity against thioacetamide-induced liver fibrosis in rats.
Female Swiss albino rats were divided into four groups: normal controls; oral administration of a sea cucumber mixed extract (14.4 mg/kg of body weight) on days 2, 4, and 6 weekly for 8 consecutive weeks; intoxication with thioacetamide (200 mg/kg of body weight, intraperitoneally) on days 2 and 6 weekly for 8 wk; and oral administration of a sea cucumber extract and then intoxication with thioacetamide 2 h later for 8 wk.
High-performance liquid chromatographic analysis of the sea cucumber mixed extract revealed the presence of some phenolic components, such as chlorogenic acid, pyrogallol, rutin, coumaric acid, catechin, and ascorbic acid. In vitro studies have shown that the extract has a high scavenging activity for the nitric oxide radical, a moderate iron-chelating activity, and a weak inhibitory effect of lipid peroxidation. The subchronic oral administration of sea cucumber extract to the rats did not show any toxic side effects but increased hepatic superoxide dismutase and glutathione peroxidase activities. The coadministration of sea cucumber extract and thioacetamide (protection modality) normalized serum direct bilirubin, alanine and aspartate aminotransferases, hepatic malondialdehyde, and hydroxyproline concentrations and antioxidant enzyme activities. In addition, the histologic examination of liver sections from the protection group that were stained with hematoxylin and eosin showed substantial attenuation of the degenerative cellular changes and regressions in liver fibrosis and necrosis induced by the thioacetamide intoxication.
Sea cucumber mixed extract contains physiologically active phenolic compounds with antioxidant activity, which afforded a potential hepatoprotective activity against thioacetamide-induced liver injury in a rat model.
通过高效液相色谱法鉴定海参（Holothuria atra）体壁混合提取物中的活性酚类化合物，并评估其对大鼠硫代乙酰胺诱导肝纤维化的保肝活性。
将雌性瑞士白化大鼠分为四组：正常对照组；每周 2、4、6 天口服海参混合提取物（14.4mg/kg 体重），连续 8 周；每周 2 和 6 天腹腔注射硫代乙酰胺（200mg/kg 体重），连续 8 周；口服海参提取物后 2 小时再腹腔注射硫代乙酰胺，连续 8 周。
高效液相色谱分析海参混合提取物发现存在一些酚类成分，如绿原酸、焦性没食子酸、芦丁、咖啡酸、儿茶素和抗坏血酸。体外研究表明，提取物对一氧化氮自由基具有高清除活性、中等铁螯合活性和弱抑制脂质过氧化作用。海参提取物的亚慢性口服给药对大鼠没有显示出任何毒性副作用，但增加了肝超氧化物歧化酶和谷胱甘肽过氧化物酶的活性。海参提取物与硫代乙酰胺（保护方式）联合给药可使血清直接胆红素、丙氨酸和天冬氨酸转氨酶、肝丙二醛和羟脯氨酸浓度以及抗氧化酶活性正常化。此外，用苏木精和伊红染色的保护组肝组织切片的组织学检查显示，硫代乙酰胺中毒引起的细胞变性变化和肝纤维化及坏死的退化明显减弱。
海参混合提取物含有具有抗氧化活性的生理活性酚类化合物，对大鼠硫代乙酰胺诱导的肝损伤具有潜在的保肝活性。
