University College London, London.
J Infect Dis. 2013 Feb 15;207(4):588-93. doi: 10.1093/infdis/jis649. Epub 2012 Oct 19.
We investigated oral fluid (OF) as an alternative to sampling of rashes for varicella zoster virus (VZV) genotyping and further characterized VZV clade prevalence in the United Kingdom and Europe. VZV was detected in up to 91% of OF specimens. Paired OF and vesicle fluid samples contained identical VZV clades. While clades 1 and 3 were the most prevalent across the United Kingdom and Europe, in Western Europe, clade 5 viruses were circulating. Viruses from the same outbreak belonged to different clades, but no clade was associated with a severe-disease phenotype. OF is suitable and convenient for large-scale molecular epidemiological studies of VZV.
我们研究了口腔液(OF)作为替代皮疹样本进行水痘带状疱疹病毒(VZV)基因分型的方法,并进一步描述了英国和欧洲 VZV 分支的流行情况。VZV 在高达 91%的 OF 标本中被检测到。配对的 OF 和水疱液样本中包含相同的 VZV 分支。虽然分支 1 和 3 在英国和欧洲最为流行,但在西欧,分支 5 的病毒正在传播。来自同一暴发的病毒属于不同的分支,但没有分支与严重疾病表型相关。OF 适用于 VZV 的大规模分子流行病学研究,方便快捷。