Service de Bactériologie-Virologie, INSERM U914, Emerging Resistance to Antibiotics, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine et Université Paris-Sud, Le Kremlin-Bicêtre, France.
Antimicrob Agents Chemother. 2013 Jan;57(1):183-8. doi: 10.1128/AAC.01384-12. Epub 2012 Oct 22.
Acinetobacter baumannii is an opportunistic pathogen that is an important source of nosocomial infections. Production of extended-spectrum β-lactamases (ESBLs) of the GES type in A. baumannii has been increasingly reported, and some of these GES-type enzymes possess some carbapenemase activity. Our aim was to analyze the resistance determinants and the clonal relationships of carbapenem-nonsusceptible A. baumannii clinical isolates recovered from hospitals in Kuwait. A total of 63 isolates were analyzed, and all were found to be positive for bla(GES)-type genes. One isolate harbored the bla(GES-14) gene encoding an ESBL with significant carbapenemase activity, whereas the other isolates harbored the bla(GES-11) ESBL gene. Thirty-three isolates coharbored the bla(OXA-23) and bla(GES-11) genes. Analyses of the genetic locations indicated that the bla(GES-11/-14) genes were plasmid located. It is noteworthy that the bla(OXA-23) and bla(GES-11) genes were colocated onto a single plasmid. Nine different pulsotypes were observed among the 63 isolates. This study showed the emergence of GES-type ESBLs in A. baumannii in Kuwait, further suggesting that the Middle East region might be a reservoir for carbapenemase-producing A. baumannii.
鲍曼不动杆菌是一种机会性病原体，是医院感染的重要来源。越来越多的报道称，鲍曼不动杆菌产生了 GES 型扩展谱β-内酰胺酶（ESBLs），其中一些 GES 型酶具有一些碳青霉烯酶活性。我们的目的是分析从科威特医院分离的耐碳青霉烯鲍曼不动杆菌临床分离株的耐药决定因子和克隆关系。共分析了 63 株分离株，所有分离株均检测到 bla(GES)-型基因阳性。一株分离株携带bla(GES-14)基因，该基因编码具有显著碳青霉烯酶活性的 ESBL，而其他分离株携带 bla(GES-11) ESBL 基因。33 株分离株共同携带 bla(OXA-23)和 bla(GES-11)基因。基因位置分析表明，bla(GES-11/-14)基因位于质粒上。值得注意的是，bla(OXA-23)和 bla(GES-11)基因位于同一质粒上。在 63 株分离株中观察到 9 种不同的脉冲场凝胶电泳图谱。本研究表明，GES 型 ESBLs 在科威特的鲍曼不动杆菌中出现，进一步表明中东地区可能是产碳青霉烯酶鲍曼不动杆菌的储存库。
