Hotter G, Roselló-Catafau J, Bulbena O, Gómez G, Colomer J, Pi F, Saenz A, Fernández Cruz L, Gelpí E
Department of Neurochemistry, Centro de Investigación y Desarrollo, CSIC, Barcelona, Spain.
Prostaglandins. 1990 Jan;39(1):53-60. doi: 10.1016/0090-6980(90)90094-c.
This work undertakes the study of changes in urinary, plasmatic and tissue levels of Thromboxane B2 (TXB2) as well as in tissue Prostaglandin E2 (PGE2) after pancreas transplantation and the effect of superoxide dismutase (SOD) on these changes. For this purpose, streptozotocine induced diabetic rats were subjected to pancreas transplantation. Experimental groups were classified as follows: Group I: Control; Group II: Animals subjected to 15 min of pancreas arterial flow occlusion followed by reperfusion; Group III: Syngenic pancreas transplantation after 12 hours of organ preservation; Group IV: Same as III, but with additional SOD (13 mg/kg) pretreatment. The results indicate that significant increases of PGE2 and TXB2 levels occur as a consequence of the surgical removal, preservation and implantation of the organ. For TXB2 these increases, immediate in plasma and tissue, are not detected in urine until 24 hours after transplantation of the pancreas. The release of TXB2 and PGE2 was effectively prevented in the SOD treated group supporting the role of oxygen free radicals and lipid peroxidation in the processes of ischemia-reperfusion associated to transplantation of the pancreas.
本研究旨在探讨胰腺移植后血栓素B2（TXB2）的尿液、血浆和组织水平以及组织前列腺素E2（PGE2）的变化，以及超氧化物歧化酶（SOD）对这些变化的影响。为此，将链脲佐菌素诱导的糖尿病大鼠进行胰腺移植。实验组分类如下：第一组：对照组；第二组：胰腺动脉血流阻断15分钟后再灌注的动物；第三组：器官保存12小时后的同基因胰腺移植；第四组：与第三组相同，但额外进行SOD（13mg/kg）预处理。结果表明，器官的手术切除、保存和植入导致PGE2和TXB2水平显著升高。对于TXB2，血浆和组织中的这些升高在胰腺移植后24小时之前在尿液中未被检测到。在SOD治疗组中，TXB2和PGE2的释放得到有效预防，这支持了氧自由基和脂质过氧化在与胰腺移植相关的缺血再灌注过程中的作用。
