Department of Laboratory Medicine, Konkuk University School of Medicine, Seoul, Republic of Korea.
Clin Biochem. 2013 Jan;46(1-2):139-42. doi: 10.1016/j.clinbiochem.2012.10.015. Epub 2012 Oct 22.
Whole blood viscosity (WBV) is the resistance of blood flow in blood vessels. Increased WBV may be a cardiovascular risk factor. The proper screening of WBV can help the early detection of cardiovascular disease. We investigated the performance of a new scanning capillary tube viscometer (SCTV) for the measurement of WBV.
We evaluated the total precision of the SCTV for 20 days using three control viscosity materials, and the within-day precision with the whole blood samples of three different individuals. For the linearity evaluation, serial dilutions of a high concentration standard material were used. For the method comparison, the results of the SCTV method were compared to those of Brookfield rotating viscometer on 227 subjects.
The SCTV had good within-run and total-run coefficient of variant (CV)s at low-, medium-, and high-concentration samples, at shear rates of 1 and 300 s(-1). The within-day CVs with the three human blood samples were 6.3%, 3.7% and 3.8% at a shear rate of 1s(-1), and 3.2%, 3.0% and 4.1% at a shear rate of 300 s(-1). The SCTV method showed an excellent linearity in the range of 84.9 to 558.2 milliPoise (mP) and 28.8 to 71.0 mP at shear rates of 1 and 300 s(-1), respectively. For the comparison study, the SCTV and the rotating viscometer showed comparable results.
The SCTV showed a stable analytical performance, and was comparable with the rotational viscometer. This new SCTV method can be used in the clinical laboratory for various needs.
全血黏度（WBV）是指血液在血管中流动的阻力。WBV 升高可能是心血管风险因素。适当的 WBV 筛查有助于早期发现心血管疾病。我们研究了一种新型扫描毛细管管黏度计（SCTV）测量 WBV 的性能。
我们使用三种控制黏度材料评估了 SCTV 连续 20 天的总精密度，并用三个不同个体的全血样本评估了日内精密度。为了评估线性，我们使用了高浓度标准物质的系列稀释液。为了方法比较，我们在 227 名受试者上比较了 SCTV 方法与 Brookfield 旋转黏度计的结果。
在低、中、高浓度样本以及剪切率为 1 和 300 s(-1)时，SCTV 在低、中、高剪切率下具有良好的日内和总运行变异系数（CV）。在剪切率为 1 s(-1)时，三个人体血液样本的日内 CV 分别为 6.3%、3.7%和 3.8%，在剪切率为 300 s(-1)时，日内 CV 分别为 3.2%、3.0%和 4.1%。SCTV 法在 84.9 至 558.2 毫泊（mP）和 1 和 300 s(-1)的剪切率分别为 28.8 至 71.0 mP 的范围内具有极好的线性。在比较研究中，SCTV 和旋转黏度计显示出可比的结果。
SCTV 显示出稳定的分析性能，与旋转黏度计相当。这种新的 SCTV 方法可用于临床实验室的各种需求。
