大鼠小肠酸性脂肪酶活性的特征及出生后的发育
Characteristics and postnatal development of the acid lipase activity of the rat small intestine.
作者信息
Coates P M, Brown S A, Jumawan J, Koldovský O
出版信息
Biochem J. 1977 Sep 15;166(3):331-8. doi: 10.1042/bj1660331.
Acid lipase was identified in the rat small intestine by using esters of 4-methylumbelliferone as substrates. Maximum activity towards the oleate ester was found at pH 4.0. In adult animals, the activity of acid lipase exhibited both latency and sedimentability, indicating a lyosomal localization. The activity of acid lipase was practically the same along the height of the villus, thus paralleling the distribution of acid beta-galactosidase. In adult rats, the activity of acid lipase in proximal (jejunum) and middle (mid-jejunum) sections of the small intestine was practically the same and exceeded the activity in the distal (ileum) section by a factor of 2. In suckling rats, the activity of the enzyme in the mid-jejunum exceeded that in the jejunum and ileum by 2.5- and 1.5-fold respectively. During postnatal development, the acid lipase activity of the mid-jejunum showed a peak between days 10 and 15, at which time it exceeded the adult mid-jejunum activity by 5--6-fold.
通过使用4-甲基伞形酮酯作为底物，在大鼠小肠中鉴定出酸性脂肪酶。在pH 4.0时发现对油酸酯的活性最高。在成年动物中，酸性脂肪酶的活性表现出潜伏期和可沉降性，表明其定位于溶酶体。沿着绒毛高度，酸性脂肪酶的活性实际上是相同的，因此与酸性β-半乳糖苷酶的分布平行。在成年大鼠中，小肠近端（空肠）和中间（空肠中部）段的酸性脂肪酶活性实际上是相同的，并且比远端（回肠）段的活性高2倍。在乳鼠中，空肠中部的酶活性分别比空肠和回肠高2.5倍和1.5倍。在出生后的发育过程中，空肠中部的酸性脂肪酶活性在第10至15天之间出现峰值，此时它比成年空肠中部的活性高5至6倍。
Zotero 插件