黑曲霉的菌株改良以提高asperenone 的产量。
Strain improvement of Aspergillus niger for the enhanced production of asperenone.
机构信息
Fermentation Technology and Bioengineering Department, Central Food Technological Research Institute, Mysore, 570 013 India.
出版信息
Indian J Microbiol. 2008 Jun;48(2):274-8. doi: 10.1007/s12088-008-0026-1. Epub 2008 Jun 13.
The enhancement of production of asperenone (Fig. 1), an inhibitor of lipoxygenase and human platelet aggregation from Aspergillus niger CFTRI 1105, was achieved by UV and nitrous acid mutagenesis. Nitrous acid mutants exhibited increased inhibitor production when compared with UV irradiated mutants. I N 41 a first-generation nitrous acid mutant produced 5.1 fold increased asperenone over parent strain. Mutant II N 31 obtained by second-generation nitrous acid treatment produced 60.3 mg asperenone/g biomass, which was 131 fold increase when compared to first generated mutant I N 41 and 670 fold increase over the parent strain. This mutant was stable for several generations on production medium.
通过 UV 和亚硝酸诱变，提高了黑曲霉 CFTRI 1105 中脂氧合酶和人血小板聚集抑制剂 Asperenone（图 1）的产量。与 UV 照射突变体相比，亚硝酸突变体表现出更高的抑制剂产量。第一代亚硝酸突变体 IN 41 的 Asperenone 产量比亲本菌株提高了 5.1 倍。通过第二代亚硝酸处理得到的突变体 II N 31 产生 60.3mg Asperenone/g 生物质，与第一代突变体 IN 41 相比提高了 131 倍，与亲本菌株相比提高了 670 倍。该突变体在生产培养基中连续几代都很稳定。
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