Environmental Physiology and Biotechnology, G B Pant Institute of Himalayan Environment and Development, Kosi-Katarmal, Almora, 263 643 India.
Indian J Microbiol. 2008 Sep;48(3):342-7. doi: 10.1007/s12088-008-0042-1. Epub 2009 Mar 25.
Bacillus megaterium strain B388, isolated from rhizosphere soil of pine belonging to a temperate Himalayan location has been characterized. The plant growth promotion and biocontrol properties of the bacterium have been evaluated through petridish and broth based assays. The isolate solubilized tricalcium phosphate under in vitro conditions; maximum activity (166 μg/ml) was recorded at 28°C after 15 days of incubation. Production of indole acetic acid demonstrated in broth assays was another important plant growth promoting character. The bacterium produced diffusible and volatile compounds that inhibited the growth of two phytopathogens viz. Alternaria alternata and Fusarium oxysporum. The carrier based formulations of the bacterium resulted in increased plant growth in bioassays. The rhizosphere colonization and the viability of the cells entrapped in alginate beads were greater in comparison to coal or broth based formulations. The bacterium showed maximum similarity with Bacillus megaterium by 16S rRNA analysis.
从属于喜马拉雅温带地区的松树根际土壤中分离到一株巨大芽孢杆菌 B388，并对其进行了鉴定。通过培养皿和肉汤基础测定，评估了该细菌的植物生长促进和生物防治特性。该分离株在体外条件下溶解磷酸三钙；在 28°C 下培养 15 天后，记录到最大活性（166μg/ml）。在肉汤测定中表现出的吲哚乙酸的产生是另一个重要的植物生长促进特性。该细菌产生可扩散和挥发性化合物，抑制两种植物病原菌（即链格孢菌和尖孢镰刀菌）的生长。基于载体的细菌制剂在生物测定中导致植物生长增加。与基于煤或肉汤的制剂相比，细菌在根际定殖和包埋在海藻酸钠珠中的细胞活力更高。通过 16S rRNA 分析，该细菌与巨大芽孢杆菌的相似度最高。
