Pindi Pavan Kumar, Sultana Tasleem, Vootla Praveen Kumar
Department of Microbiology, Palamuru University, Mahabubnagar, 509001, Andhra Pradesh, India.
3 Biotech. 2014 Jun;4(3):305-315. doi: 10.1007/s13205-013-0154-0. Epub 2013 Jul 20.
Deccan plateau in India periodically experiences droughts due to irregular rain fall and the soil in many parts of the region is considered to be poor for farming. Plant growth promoting rhizobacteria are originally defined as root-colonizing bacteria, i.e., Bacillus that cause either plant growth promotion or biological control of plant diseases. The study aims at the isolation of novel Bacillus species and to assess the biotechnological potential of the novel species as a biofertilizer, with respect to their plant growth promoting properties as efficient phosphate-solubilizing bacteria. Seven different strains of Bacillus were isolated from cotton rhizosphere soil near boys' hostel of Palamuru University which belongs to Deccan plateau. Among seven isolated strains, Bacillus strain-7 has shown maximum support for good growth of eight cotton cultivars. This bacterial species is named Bacillus sp. PU-7 based on the phenotypic and phylogenetic analysis. Among eight cotton cultivars, Mahyco has shown high levels of IAA, proteins, chlorophyll, sugars and low level of proline. Efficacy of novel Bacillus sp. PU-7 with Mahyco cultivar has been checked experimentally at field level in four different cotton grown agricultural soils. The strains supported plant growth in almost all the cases, especially in the deep black soil, with a clear evidence of maximum plant growth by increased levels of phytohormone production and biochemical analysis, followed by shallow black soil. Hence, it is inferred that the novel isolate can be used as bioinoculant in the cotton fields.
印度的德干高原由于降雨不规则，时常遭受旱灾，该地区许多地方的土壤被认为不适宜耕种。植物促生根际细菌最初被定义为定殖于根部的细菌，即能促进植物生长或对植物病害进行生物防治的芽孢杆菌。本研究旨在分离新型芽孢杆菌物种，并评估这些新物种作为生物肥料的生物技术潜力，考察其作为高效解磷细菌促进植物生长的特性。从位于德干高原的帕拉穆鲁大学男生宿舍附近的棉花根际土壤中分离出7种不同的芽孢杆菌菌株。在这7种分离菌株中，芽孢杆菌菌株-7对8个棉花品种的良好生长表现出最大的促进作用。基于表型和系统发育分析，将该细菌物种命名为芽孢杆菌PU-7。在8个棉花品种中，Mahyco的吲哚乙酸、蛋白质、叶绿素、糖分含量较高，脯氨酸含量较低。在4种不同种植棉花的农业土壤田间水平上，对新型芽孢杆菌PU-7与Mahyco品种的效果进行了实验检验。在几乎所有情况下，这些菌株都能促进植物生长，尤其是在深黑土中，通过植物激素产量增加和生化分析，有明确证据表明植物生长达到最大值，其次是浅黑土。因此，可以推断这种新分离菌株可作为棉田生物接种剂。
