抗T3自身抗体对血清中总T3和游离T3测定的干扰。
Interference of anti-T3 autoantibodies in the measurement of total and free T3 in serum.
作者信息
Abraham R, Chawla R
机构信息
Department of Biochemistry, Christian Medical College & Hospital, 141008 Ludhiana (Punjab).
出版信息
Indian J Clin Biochem. 1997 Dec;12(1):40-4. doi: 10.1007/BF02867953.
A case of anti-trilodothyronine autoantibodies is presented in this report. The thyroid hormone profile of a hypertensive patient, with multinodular goiter and history of exogenous thyroid hormone therapy, was found to be highly ambiguous. The total as well as free T3 levels were consistently high (out of range) whereas the T4 (total and free) values were always within normal limits. The thyrotropin was found to be partially suppressed. Very low T3-Uptake indicated some kind of interference in the immunoassays. We incubated the sera with the radio-iodine labelled T3 and observed that the patient's serum bound about ten times more radioactivity than a control run in parallel. On further resolving the serum proteins on cellulose acetate electrophoresis, the radioactivity was detected in the γ-globulin band. Therefore it was established that the patient's serum carried the antibodies reactive with T3 which were interfering in the immunoassays. Elevated anti-thyroid microsomal antibodies were also present in the patient's serum. The anti-T3 antibodies were highly specific for T3 and did not show any cross reactivity with the T4 or its analogues used in the estimation of free T4.
本报告介绍了一例抗三碘甲状腺原氨酸自身抗体的病例。一名患有多结节性甲状腺肿且有外源性甲状腺激素治疗史的高血压患者，其甲状腺激素谱情况非常不明确。总三碘甲状腺原氨酸（T3）水平以及游离T3水平一直很高（超出范围），而甲状腺素（T4，总T4和游离T4）值始终在正常范围内。促甲状腺激素被发现受到部分抑制。极低的T3摄取率表明免疫测定存在某种干扰。我们将血清与放射性碘标记的T3一起孵育，观察到患者血清结合的放射性比平行进行的对照高出约十倍。在醋酸纤维素电泳上进一步分离血清蛋白时，在γ-球蛋白带中检测到放射性。因此确定患者血清中携带与T3反应的抗体，这些抗体干扰了免疫测定。患者血清中还存在抗甲状腺微粒体抗体升高的情况。抗T3抗体对T3具有高度特异性，对用于估算游离T4的T4或其类似物未显示任何交叉反应性。
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