Department of Physics, Duke University, Durham, North Carolina 27708, USA.
Nano Lett. 2012 Nov 14;12(11):5936-40. doi: 10.1021/nl303358p. Epub 2012 Oct 30.
Nonlinear optical microscopy, based on femtosecond laser spectral reshaping, characterized and imaged graphene samples made from different methods, both on slides and in a biological environment. This technique clearly discriminates between graphene flakes with different numbers of layers and reveals the distinct nonlinear optical properties of reduced graphene oxide as compared to mechanically exfoliated or chemical vapor deposition grown graphene. The nonlinearity makes it applicable to scattering samples (such as tissue) as opposed to previous methods, such as transmission. This was demonstrated by high-resolution imaging of breast cancer cells incubated with graphene flakes.
基于飞秒激光光谱整形的非线性光学显微镜,对不同方法制备的石墨烯样品进行了特征成像和分析,包括在玻片上和生物环境中。该技术可明确区分具有不同层数的石墨烯薄片,并显示还原氧化石墨烯与机械剥离或化学气相沉积生长的石墨烯相比具有独特的非线性光学性质。与以往的透射方法相比,该非线性光学性质使得该技术适用于散射样品(如组织)。这一点通过用石墨烯薄片孵育的乳腺癌细胞的高分辨率成像得到了证明。