有证据表明，在冷适应大鼠的肩胛间棕色脂肪组织中，脂肪酸合成在体内会因胰岛素而增加，其机制涉及丙酮酸脱氢酶和乙酰辅酶A羧化酶的平行激活。
Evidence that fatty acid synthesis in the interscapular brown adipose tissue of cold-adapted rats is increased in vivo by insulin by mechanisms involving parallel activation of pyruvate dehydrogenase and acetyl-coenzyme A carboxylase.
作者信息
McCormack J G, Denton R M
出版信息
Biochem J. 1977 Sep 15;166(3):627-30. doi: 10.1042/bj1660627.
Abstract
Plasma insulin concentrations in cold-adapted rats were altered acutely by administration of glucose or anti-insulin serum. Rates of fatty acid synthesis in interscapular brown adipose tissue were determined from the incorporation of 3H from 3H2O into tissue lipid. Rates of synthesis were greatly elevated after glucose administration and markedly decreased after injection with anti-insulin serum. Parallel changes in the initial activities of both acetyl-CoA carboxylase and pyruvate dehydrogenase were observed under these conditions, but no changes in total activities were evident. The results suggest that this tissue is an important site of fatty acid synthesis in the cold-adapted rat and that this feature of the tissue is sensitive to changes in plasma insulin concentrations.
摘要
给予葡萄糖或抗胰岛素血清可使冷适应大鼠的血浆胰岛素浓度发生急性改变。通过将3H2O中的3H掺入组织脂质中来测定肩胛间棕色脂肪组织中脂肪酸合成的速率。给予葡萄糖后合成速率大幅升高，注射抗胰岛素血清后合成速率显著降低。在这些条件下，观察到乙酰辅酶A羧化酶和丙酮酸脱氢酶的初始活性有平行变化，但总活性没有明显变化。结果表明，该组织是冷适应大鼠脂肪酸合成的重要部位，并且该组织的这一特性对血浆胰岛素浓度的变化敏感。
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