抗胰岛素血清对喂食大鼠肝脏和附睾脂肪组织中脂肪酸合成速率、乙酰辅酶A羧化酶及丙酮酸脱氢酶活性的体内急性效应
Acute effects in vivo of anti-insulin serum on rates of fatty acid synthesis and activities of acetyl-coenzyme A carboxylase and pyruvate dehydrogenase in liver and epididymal adipose tissue of fed rats.
作者信息
Stansbie D, Brownsey R W, Crettaz M, Denton R M
出版信息
Biochem J. 1976 Nov 15;160(2):413-6. doi: 10.1042/bj1600413.
Abstract
Plasma insulin concentrations in fed rats were altered acutely by administration of glucose or anti-insulin serum. Rates of fatty acid synthesis in adipose tissue and liver were estimated from the incorporation of 3H from 3H2O. In the adipose tissue dehydrogenase and acetyl-CoA carboxylase were evident. In liver, although changes in rates of fatty acid synthesis were found, the initial activity of pyruvate dehydrogenase did not alter, but small parallel changes in acetyl-CoA carboxylase activity were observed.
摘要
给进食的大鼠注射葡萄糖或抗胰岛素血清后，其血浆胰岛素浓度会迅速改变。通过测定3H2O中3H的掺入量来估算脂肪组织和肝脏中脂肪酸的合成速率。在脂肪组织中，脱氢酶和乙酰辅酶A羧化酶很明显。在肝脏中，虽然发现脂肪酸合成速率有变化，但丙酮酸脱氢酶的初始活性并未改变，不过观察到乙酰辅酶A羧化酶活性有小的平行变化。
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