Institute of Terrestrial Ecosystems (ITES)-Ecosystem Management, Building CHN Floor G, ETH Zürich, Universitaetstrasse 16, 8092 Zürich, Switzerland.
Am J Bot. 2012 Nov;99(11):e447-9. doi: 10.3732/ajb.1200102. Epub 2012 Oct 29.
Microsatellite markers were developed in Trifolium montanum to investigate pollen dispersal patterns at landscape scale with a pollen-pool analysis (indirect parentage analysis) as well as in an experimental set-up with a direct paternity analysis.
Screening 46 microsatellites developed for T. repens yielded four markers usable in T. montanum. Seven additional ones have been developed specifically for the target species using a 454-sequencing approach. All markers were polymorphic, with an allele number ranging from two to 45 based upon 254 individuals sampled from four populations, and an exclusion probability of 0.999.
These markers proved a useful and reliable molecular tool for use in population genetics and parentage studies of this common grassland herb.
开发三叶草属的微卫星标记，通过花粉池分析（间接亲子分析）以及实验设置进行直接亲子鉴定，研究景观尺度上的花粉扩散模式。
筛选出可用于三叶草的 46 个微卫星标记，其中 4 个标记在高山三叶草中可用。另外 7 个标记是专门针对目标物种使用 454 测序方法开发的。所有标记均表现出多态性，在从四个种群中采集的 254 个个体中，等位基因数量从两个到 45 不等，排除概率为 0.999。
这些标记为该常见草原草本植物的种群遗传学和亲子关系研究提供了一种有用且可靠的分子工具。
