Department of Orthopedic Surgery, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310009, China.
Knee Surg Sports Traumatol Arthrosc. 2014 Jun;22(6):1424-33. doi: 10.1007/s00167-012-2256-3. Epub 2012 Oct 30.
The integration of regenerated cartilage with surrounding native cartilage is a major challenge for the success of cartilage tissue-engineering strategies. The purpose of this study is to investigate whether incorporation of the power of mesenchymal stem cell (MSC) sheet to MSCs-loaded bilayer poly-(lactic-co-glycolic acid) (PLGA) scaffolds can improve the integration and repair of cartilage defects in a rabbit model.
Rabbit bone marrow-derived MSCs were cultured and formed cell sheet. Full-thickness cylindrical osteochondral defects (4 mm in diameter, 3 mm in depth) were created in the patellar groove of 18 New Zealand white rabbits and the osteochondral defects were treated with PLGA scaffold (n = 6), PLGA/MSCs (n = 6) or MSC sheet-encapsulated PLGA/MSCs (n = 6). After 6 and 12 weeks, the integration and tissue response were evaluated histologically.
The MSC sheet-encapsulated PLGA/MCSs group showed significantly more amounts of hyaline cartilage and higher histological scores than PLGA/MSCs group and PLGA group (P < 0.05). In addition, the MSC sheet-encapsulated PLGA/MCSs group showed the best integration between the repaired cartilage and surrounding normal cartilage and subchondral bone compared to other two groups.
The novel method of incorporation of MSC sheet to PLGA/MCSs could enhance the ability of cartilage regeneration and integration between repair cartilage and the surrounding cartilage. Transplantation of autologous MSC sheet combined with traditional strategies or cartilage debris might provide therapeutic opportunities for improving cartilage regeneration and integration in humans.
再生软骨与周围天然软骨的整合是软骨组织工程策略成功的主要挑战。本研究旨在探讨将间充质干细胞（MSC）片的力量纳入负载 MSC 的双层聚（乳酸-共-乙醇酸）（PLGA）支架是否可以改善兔模型中软骨缺损的整合和修复。
培养兔骨髓来源的 MSC 并形成细胞片。在 18 只新西兰白兔的髌沟中创建全层圆柱形骨软骨缺损（直径 4mm，深度 3mm），并用 PLGA 支架（n=6）、PLGA/MSCs（n=6）或 MSC 片包裹的 PLGA/MSCs（n=6）处理骨软骨缺损。6 周和 12 周后，通过组织学评估评估整合和组织反应。
MSC 片包裹的 PLGA/MCSs 组的透明软骨量和组织学评分明显高于 PLGA/MSCs 组和 PLGA 组（P<0.05）。此外，与其他两组相比，MSC 片包裹的 PLGA/MCSs 组在修复的软骨与周围正常软骨和软骨下骨之间表现出最佳的整合。
将 MSC 片纳入 PLGA/MCSs 的新方法可以增强软骨再生和修复软骨与周围软骨之间整合的能力。自体 MSC 片与传统策略或软骨碎片的移植可能为改善人类软骨再生和整合提供治疗机会。
