Warwick Systems Biology Centre, University of Warwick, Coventry CV4 7AL, United Kingdom.
Plant Cell. 2012 Oct;24(10):3949-65. doi: 10.1105/tpc.112.103010. Epub 2012 Oct 30.
Conserved noncoding sequences (CNSs) in DNA are reliable pointers to regulatory elements controlling gene expression. Using a comparative genomics approach with four dicotyledonous plant species (Arabidopsis thaliana, papaya [Carica papaya], poplar [Populus trichocarpa], and grape [Vitis vinifera]), we detected hundreds of CNSs upstream of Arabidopsis genes. Distinct positioning, length, and enrichment for transcription factor binding sites suggest these CNSs play a functional role in transcriptional regulation. The enrichment of transcription factors within the set of genes associated with CNS is consistent with the hypothesis that together they form part of a conserved transcriptional network whose function is to regulate other transcription factors and control development. We identified a set of promoters where regulatory mechanisms are likely to be shared between the model organism Arabidopsis and other dicots, providing areas of focus for further research.
DNA 中的保守非编码序列 (CNSs) 是控制基因表达的调控元件的可靠指针。我们使用四种双子叶植物（拟南芥、木瓜、杨树和葡萄）的比较基因组学方法，检测到了数百个位于拟南芥基因上游的 CNSs。这些 CNSs 在位置、长度和转录因子结合位点的富集方面存在明显差异，表明它们在转录调控中具有功能作用。与 CNS 相关的基因中富集的转录因子与这样一个假设一致，即它们共同构成了一个保守的转录网络的一部分，其功能是调节其他转录因子并控制发育。我们确定了一组启动子，其中调控机制可能在模式生物拟南芥和其他双子叶植物之间共享，为进一步的研究提供了重点关注的领域。
