Malchiodi Albedi F, Cassano A M, Ciaralli F, Taruscio D, Giuliani A, Donelli G
Laboratorio di Ultrastrutture, Istituto Superiore di Sanità, Roma, Italia.
J Submicrosc Cytol Pathol. 1990 Jan;22(1):153-8.
The ultrastructural expression of laminin and fibronectin was studied in human colonic mucosa with a pre-embedding immunoperoxidase technique. Both antigens were present in epithelial basement membrane. The lamina densa was uniformly and homogeneously immunostained by both fibronectin and laminin, while the lamina rara showed discrete, positive sites occasionally connecting the lamina densa with the epithelial plasma membrane. In the lamina reticularis, fibronectin was diffusely positive, while laminin showed a discontinuous positivity only in the uppermost region in vicinity of the lamina densa. Collagen fibrils were regularly decorated by fibronectin-positive material. Basement membranes in the capillary wall showed a similar pattern of reactivity both with laminin and fibronectin. These results point out that laminin as well as fibronectin are integral components of intestinal mucosa basement membranes.
采用包埋前免疫过氧化物酶技术研究了层粘连蛋白和纤连蛋白在人结肠黏膜中的超微结构表达。两种抗原均存在于上皮基底膜中。致密层被纤连蛋白和层粘连蛋白均匀且一致地免疫染色，而疏松层显示出离散的阳性位点，偶尔将致密层与上皮质膜相连。在网状层中，纤连蛋白呈弥漫性阳性，而层粘连蛋白仅在致密层附近的最上部区域呈间断阳性。胶原纤维被纤连蛋白阳性物质规则地修饰。毛细血管壁的基底膜与层粘连蛋白和纤连蛋白的反应模式相似。这些结果表明，层粘连蛋白和纤连蛋白都是肠黏膜基底膜的组成成分。
