电穿孔法 DNA 疫苗：您应该了解的知识。
What you always needed to know about electroporation based DNA vaccines.
机构信息
Center for Experimental Drug and Gene Electrotransfer (C*EDGE), Department of Oncology, Copenhagen University Hospital Herlev, Herlev, Denmark.
出版信息
Hum Vaccin Immunother. 2012 Nov 1;8(11):1694-702. doi: 10.4161/hv.22062. Epub 2012 Oct 30.
Abstract
Vaccinations are increasingly used to fight infectious disease, and DNA vaccines offer considerable advantages, including broader possibilities for vaccination and lack of need for cold storage. It has been amply demonstrated, that electroporation augments uptake of DNA in both skin and muscle, and it is foreseen that future DNA vaccination may to a large extent be coupled with and dependent upon electroporation based delivery. Understanding the basic science of electroporation and exploiting knowledge obtained on optimization of DNA electrotransfer to muscle and skin, may greatly augment efforts on vaccine development. The purpose of this review is to give a succinct but comprehensive overview of electroporation as a delivery modality including electrotransfer to skin and muscle. As well, this review will speculate and discuss future uses for this powerful electrotransfer technology.
摘要
疫苗接种越来越多地被用于对抗传染病，而 DNA 疫苗具有相当大的优势，包括更广泛的接种可能性和无需冷藏。已经充分证明，电穿孔增强了皮肤和肌肉中 DNA 的摄取，预计未来的 DNA 疫苗接种在很大程度上将与基于电穿孔的递送相结合并依赖于它。了解电穿孔的基础科学，并利用在优化 DNA 电转移到肌肉和皮肤方面获得的知识，可以极大地推动疫苗开发工作。本文综述的目的是简要而全面地概述电穿孔作为一种传递方式，包括电转移到皮肤和肌肉。此外，本文还将推测和讨论这种强大的电转移技术的未来用途。
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