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呼吸道感染患者中耶氏肺孢子菌的分子检测

Molecular detection of pneumocystis jirovecii in patients with respiratory tract infections.

作者信息

Alli Oyebode Armstrong Terry, Ogbolu David Olusoga, Ademola Olufunmi, Oyenike Musiliu Adewale

机构信息

Department of Biomedical Sciences, College of Health Sciences, Ladoke Akintola University of Technology, Osogbo, Nigeria.

出版信息

N Am J Med Sci. 2012 Oct;4(10):479-85. doi: 10.4103/1947-2714.101993.

DOI:10.4103/1947-2714.101993
PMID:23112970
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3482780/
Abstract

BACKGROUND

Pneumocystis jirovecii, formerly known as P. carinii, is an opportunistic fungus causing Pneumocystis carinii pneumonia especially in immunocompromised patients.

AIM

The aim of this study was to detect P. jirovecii in sputum samples from patients suspected of having respiratory tract infections.

MATERIALS AND METHODS

In this study, 230 acid fast bacilli negative sputum samples from 230 patients presenting with respiratory tract infections submitted to three teaching hospitals' medical microbiology laboratories in Osun and Oyo States, Nigeria for routine investigation were examined for P. jirovecii by microscopical and polymerase chain reaction methods.

RESULTS

P. jirovecii cysts were observed in 15 (6.5%) samples and polymerase chain reaction was positive for 29 (12.6%) samples out of 230 samples examined. It was observed that the detection of P. jirovecii was associated with age (P < 0.05) while there were no associations between diagnosis, sex, and prevalence of P. jirovecii (P > 0.05). Polymerase chain reaction was showed to be a better method for the detection of P. jirovecii based on the 51.7% sensitivity and 100% specificity of the microscopy.

CONCLUSION

The study concluded that P. jirovecii is prevalent in patients with respiratory tract infections in hospitals from the southwestern part of Nigeria and should be included in diagnosis of these infections in this part of the world.

摘要

背景

耶氏肺孢子菌，以前称为卡氏肺孢子菌，是一种机会性真菌，可引起卡氏肺孢子菌肺炎，尤其是在免疫功能低下的患者中。

目的

本研究的目的是检测疑似呼吸道感染患者痰液样本中的耶氏肺孢子菌。

材料与方法

在本研究中，对尼日利亚奥孙州和奥约州三家教学医院医学微生物实验室送检的230例呼吸道感染患者的230份抗酸杆菌阴性痰液样本进行常规检查，采用显微镜检查和聚合酶链反应方法检测耶氏肺孢子菌。

结果

在230份检测样本中，15份（6.5%）样本观察到耶氏肺孢子菌囊肿，29份（12.6%）样本聚合酶链反应呈阳性。观察发现，耶氏肺孢子菌的检测与年龄有关（P<0.05），而诊断、性别与耶氏肺孢子菌患病率之间无关联（P>0.05）。基于显微镜检查51.7%的敏感性和100%的特异性，聚合酶链反应被证明是检测耶氏肺孢子菌的更好方法。

结论

该研究得出结论，耶氏肺孢子菌在尼日利亚西南部医院的呼吸道感染患者中普遍存在，在世界这一地区的这些感染诊断中应包括该菌。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd89/3482780/cc0ef98a6fbc/NAJMS-4-479-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd89/3482780/437d90cb9338/NAJMS-4-479-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd89/3482780/bd02dd9c486d/NAJMS-4-479-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd89/3482780/643757a3be8d/NAJMS-4-479-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd89/3482780/cc0ef98a6fbc/NAJMS-4-479-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd89/3482780/437d90cb9338/NAJMS-4-479-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd89/3482780/bd02dd9c486d/NAJMS-4-479-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd89/3482780/643757a3be8d/NAJMS-4-479-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd89/3482780/cc0ef98a6fbc/NAJMS-4-479-g004.jpg

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