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The Brief Case: False-negative PCR.病例摘要：聚合酶链反应假阴性
J Clin Microbiol. 2024 Jul 16;62(7):e0009424. doi: 10.1128/jcm.00094-24.
2
Detection of Pneumocystis carinii DNA in blood by PCR is not of value for diagnosis of P. carinii pneumonia.通过聚合酶链反应（PCR）检测血液中卡氏肺孢子虫DNA对卡氏肺孢子虫肺炎的诊断没有价值。
J Clin Microbiol. 1996 Jun;34(6):1586-8. doi: 10.1128/jcm.34.6.1586-1588.1996.
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Recent Advances in the Diagnosis of Pneumocystis Pneumonia.肺孢子菌肺炎诊断的最新进展
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Pneumocystis polymerase chain reaction and blood (1→3)-β-D-glucan assays to predict survival with suspected Pneumocystis jirovecii pneumonia.肺孢子菌聚合酶链反应及血液（1→3）-β-D-葡聚糖检测对疑似耶氏肺孢子菌肺炎患者生存情况的预测价值
J Infect Chemother. 2014 Feb;20(2):109-14. doi: 10.1016/j.jiac.2013.09.004. Epub 2013 Dec 11.
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Noninvasive method for monitoring Pneumocystis carinii pneumonia.监测卡氏肺孢子虫肺炎的非侵入性方法。
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[Evaluation of a panfungal pcr assay for the diagnosis of pneumocystis carinii pneumonia (pcp)].[用于诊断卡氏肺孢子虫肺炎（PCP）的泛真菌聚合酶链反应检测法的评估]
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Comparison between real-time PCR, conventional PCR and different staining techniques for diagnosing Pneumocystis jiroveci pneumonia from bronchoalveolar lavage specimens.实时聚合酶链反应、传统聚合酶链反应与不同染色技术在通过支气管肺泡灌洗标本诊断耶氏肺孢子菌肺炎中的比较。
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Comparison of single and touchdown PCR protocols for detecting Pneumocystis jirovecii DNA in paraffin-embedded lung tissue samples.用于检测石蜡包埋肺组织样本中耶氏肺孢子菌DNA的单重PCR与降落PCR方案比较
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A highly sensitive novel PCR assay for detection of Pneumocystis jirovecii DNA in bronchoalveloar lavage specimens from immunocompromised patients.一种高敏感的新型 PCR 检测方法，用于检测免疫抑制患者支气管肺泡灌洗液中的肺孢子菌 DNA。
Clin Microbiol Infect. 2012 Jun;18(6):598-603. doi: 10.1111/j.1469-0691.2011.03656.x. Epub 2011 Sep 26.

本文引用的文献

1
Comparison of the PneumoGenius® and RealStar® Pneumocystis jirovecii PCR CE-IVD assays with a lab developed test for the detection of Pneumocystis jirovecii.比较 PneumoGenius® 和 RealStar® 肺孢子菌 PCR CE-IVD 检测试剂盒与实验室自建检测方法用于检测肺孢子菌。
Med Mycol. 2022 Jan 10;60(1). doi: 10.1093/mmy/myab080.
2
Pneumocystis jirovecii Disease: Basis for the Revised EORTC/MSGERC Invasive Fungal Disease Definitions in Individuals Without Human Immunodeficiency Virus.肺孢子菌病：修订后的 EORTC/MSGERC 侵袭性真菌病定义在无人类免疫缺陷病毒个体中的基础。
Clin Infect Dis. 2021 Mar 12;72(Suppl 2):S114-S120. doi: 10.1093/cid/ciaa1805.
3
Specificity Influences in (1→3)-β-d-Glucan-Supported Diagnosis of Invasive Fungal Disease.特异性对（1→3）-β-D-葡聚糖在侵袭性真菌病诊断中的影响
J Fungi (Basel). 2020 Dec 29;7(1):14. doi: 10.3390/jof7010014.
4
Diagnosing Pneumocystis jirovecii pneumonia: A review of current methods and novel approaches.诊断耶氏肺孢子菌肺炎：当前方法与新途径综述
Med Mycol. 2020 Nov 10;58(8):1015-1028. doi: 10.1093/mmy/myaa024.
5
The Fungal PCR Initiative's evaluation of in-house and commercial Pneumocystis jirovecii qPCR assays: Toward a standard for a diagnostics assay.真菌 PCR 倡议对内部和商业的肺孢子菌 jirovecii qPCR 检测方法的评估：朝着诊断检测方法的标准化迈进。
Med Mycol. 2020 Aug 1;58(6):779-788. doi: 10.1093/mmy/myz115.
6
PCR: It Is Time to Make PCR the Test of Choice.聚合酶链反应（PCR）：是时候让PCR成为首选检测方法了。
Open Forum Infect Dis. 2017 Sep 2;4(4):ofx193. doi: 10.1093/ofid/ofx193. eCollection 2017 Fall.
7
Copy Number Variation of Mitochondrial DNA Genes in Pneumocystis jirovecii According to the Fungal Load in BAL Specimens.根据支气管肺泡灌洗标本中的真菌载量分析耶氏肺孢子菌线粒体DNA基因的拷贝数变异
Front Microbiol. 2016 Sep 12;7:1413. doi: 10.3389/fmicb.2016.01413. eCollection 2016.
8
Pneumocystis pneumonia in HIV patients: a diagnostic challenge till date.HIV患者的肺孢子菌肺炎：迄今为止的诊断挑战。
Med Mycol. 2015 Aug;53(6):587-92. doi: 10.1093/mmy/myv023.
9
Molecular detection of pneumocystis jirovecii in patients with respiratory tract infections.呼吸道感染患者中耶氏肺孢子菌的分子检测
N Am J Med Sci. 2012 Oct;4(10):479-85. doi: 10.4103/1947-2714.101993.
10
Guidelines for prevention and treatment of opportunistic infections in HIV-infected adults and adolescents: recommendations from CDC, the National Institutes of Health, and the HIV Medicine Association of the Infectious Diseases Society of America.《HIV 感染成人及青少年机会性感染的预防与治疗指南：美国疾病控制与预防中心、国立卫生研究院及美国传染病学会 HIV 医学协会的建议》
MMWR Recomm Rep. 2009 Apr 10;58(RR-4):1-207; quiz CE1-4.

The Brief Case: False-negative PCR.

作者信息

Rattin Jacob, Marigney Kelly, Miranda Cyndee, Arrossi A Valeria, Misra Anisha, Wang Hannah

机构信息

Department of Laboratory Medicine, Cleveland Clinic, Cleveland, Ohio, USA.

Department of Pathology, Cleveland Clinic, Cleveland, Ohio, USA.

出版信息

J Clin Microbiol. 2024 Jul 16;62(7):e0009424. doi: 10.1128/jcm.00094-24.

DOI:10.1128/jcm.00094-24
PMID:39012140
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11250520/
Abstract
摘要