Nazarova G A, Kolyasnikova K N, Zolotov N N
PK Anokhin Institute of Normal Physiology, Russian Academy of Medical Sciences, VV Zakusov Institute of Pharmacology, Russian Academy of Medical Sciences, Moscow, Russia.
Bull Exp Biol Med. 2012 Sep;153(5):674-6. doi: 10.1007/s10517-012-1796-3.
We studied the role of proline-specific peptidases in the pathogenesis of Alzheimer's disease. Testing of conditioned passive avoidance 24 h after learning showed that chronic administration of scopolamine to rats 4-fold reduced the latency of entry into the dark chamber in comparison with controls (intact animals). Activity of prolyl endopeptidase was significantly higher than in the controls in both the cortex and hippocampus. Changes in dipeptidyl peptidase IV activity were observed only in the cortex. Injection of AF-64A toxin into Meynert nucleus basalis reduced the latency of entry into the dark compartment by 75% in comparison with that in sham-operated and intact controls. Prolyl endopeptidase activity was reduced in the frontal cortex and hippocampus, but not in hypothalamus. Changes in dipeptidyl peptidase IV activity were detected only in the frontal cortex.
我们研究了脯氨酸特异性肽酶在阿尔茨海默病发病机制中的作用。学习后24小时进行的条件性被动回避测试表明，与对照组（未处理动物）相比，向大鼠长期注射东莨菪碱使进入暗室的潜伏期缩短了4倍。脯氨酰内肽酶的活性在皮质和海马体中均显著高于对照组。仅在皮质中观察到二肽基肽酶IV活性的变化。与假手术组和未处理对照组相比，向基底核Meynert核注射AF - 64A毒素使进入暗室的潜伏期缩短了75%。额叶皮质和海马体中的脯氨酰内肽酶活性降低，但下丘脑未出现这种情况。仅在额叶皮质中检测到二肽基肽酶IV活性的变化。
