Department of Materials Engineering, University of British Columbia, 309-6350 Stores Road, Vancouver, BC, V6T 1Z4, Canada.
J Mater Sci Mater Med. 2013 Feb;24(2):355-64. doi: 10.1007/s10856-012-4794-0. Epub 2012 Nov 1.
A novel calcium phosphate silicate bone cement (CPSC) was synthesized in a process, in which nanocomposite forms in situ between calcium silicate hydrate (C-S-H) gel and hydroxyapatite (HAP). The cement powder consists of tricalcium silicate (C(3)S) and calcium phosphate monobasic (CPM). During cement setting, C(3)S hydrates to produce C-S-H and calcium hydroxide (CH); CPM reacts with the CH to precipitate HAP in situ within C-S-H. This process, largely removing CH from the set cement, enhances its biocompatibility and bioactivity. The testing results of cell culture confirmed that the biocompatibility of CPSC was improved as compared to pure C(3)S. The results of XRD and SEM characterizations showed that CPSC paste induced formation of HAP layer after immersion in simulated body fluid for 7 days, suggesting that CPSC was bioactive in vitro. CPSC cement, which has good biocompatibility and low/no cytotoxicity, could be a promising candidate as biomedical cement.
一种新型的磷酸钙硅酸盐骨水泥（CPSC）是通过一种工艺合成的，该工艺在硅酸钙水合物（C-S-H）凝胶和羟基磷灰石（HAP）之间原位形成纳米复合材料。水泥粉末由硅酸三钙（C3S）和磷酸一钙（CPM）组成。在水泥凝固过程中，C3S 水化生成 C-S-H 和氢氧化钙（CH）；CPM 与 CH 反应，在 C-S-H 内原位沉淀 HAP。这一过程从凝固的水泥中大量去除 CH，提高了其生物相容性和生物活性。细胞培养的测试结果证实，与纯 C3S 相比，CPSC 的生物相容性得到了提高。XRD 和 SEM 特性的结果表明，CPSC 糊剂在模拟体液中浸泡 7 天后诱导形成 HAP 层，表明 CPSC 在体外具有生物活性。CPSC 水泥具有良好的生物相容性和低/无细胞毒性，有望成为一种有前途的生物医学水泥。
