Department of Biotechnology, Alagappa University, Karaikudi, Tamil Nadu, India.
Planta Med. 2012 Dec;78(18):1918-26. doi: 10.1055/s-0032-1327879. Epub 2012 Oct 31.
Dendrophthoe falcata is a hemiparasitic plant commonly used for ailments such as ulcers, asthma, impotence, paralysis, skin diseases, menstrual troubles, pulmonary tuberculosis, and wounds. In this context, the validations of the traditional claim that the leaf extract of D. falcata possesses antibiofilm and anti-quorum sensing activity against different bacterial pathogens were assessed. The bacterial biofilms were quantified by crystal violet staining. Among the 17 bacterial pathogens screened, the methanolic fraction of the leaf extract clearly demonstrated antibiofilm activity for Proteus mirabilis, Vibrio vulnificus, Aeromonas hydrophila, Shigella sonnei, Chromobacterium violaceum ATCC 12472, Vibrio parahaemolyticus, Vibrio harveyi, Vibrio alginolyticus, Vibrio cholerae, and Proteus vulgaris. At biofilm inhibitory concentrations, biofilm formation was reduced by up to 70-90 %. Furthermore, the potential quorum-sensing activity of the leaf extract was tested by agar well diffusion using Chromobacterium violaceum (ATCC 12472 & CV O26) reporter strains. The inhibition of violacein production may be due to direct or indirect interference on QS by active constituents or the interactive effect of different phytocompounds present in the extracts. This is the first report on antibiofilm and QS activity of D. falcata leaf extracts, signifying the scope for development of complementary medicine for biofilm-associated infections.
杠柳是一种半寄生植物，常用于治疗溃疡、哮喘、阳痿、瘫痪、皮肤病、月经失调、肺结核和创伤等疾病。在这种情况下，评估了杠柳叶提取物具有抗生物膜和抗群体感应活性的传统说法的有效性，以对抗不同的细菌病原体。通过结晶紫染色来定量细菌生物膜。在筛选出的 17 种细菌病原体中，杠柳叶的甲醇提取物对奇异变形杆菌、创伤弧菌、嗜水气单胞菌、宋内志贺菌、紫色色杆菌 ATCC 12472、副溶血性弧菌、哈维氏弧菌、Alginate 弧菌、霍乱弧菌和普通变形杆菌表现出明显的抗生物膜活性。在生物膜抑制浓度下，生物膜形成减少了 70-90%。此外，还通过使用紫色色杆菌（ATCC 12472 和 CV O26）报告菌株的琼脂孔扩散法测试了叶提取物的潜在群体感应活性。紫色素产生的抑制可能是由于活性成分对 QS 的直接或间接干扰，或提取物中存在的不同植物化合物的相互作用。这是杠柳叶提取物具有抗生物膜和 QS 活性的首次报道，为生物膜相关感染的补充医学开发提供了依据。
