Noumi Emira, Snoussi Mejdi, Merghni Abderrahmen, Nazzaro Filomena, Quindós Guillermo, Akdamar Gültekin, Mastouri Maha, Al-Sieni Abdulbasset, Ceylan Ozgur
Laboratory of Transmissible Diseases and Biologically Active Substances, Faculty of Pharmacy, University of Monastir, Tunisia.
Laboratory of Treatment and Valorization of Water Discharges (CERTE, Borj Cedria), Soliman 8020, Tunisia.
Microb Pathog. 2017 Aug;109:169-176. doi: 10.1016/j.micpath.2017.05.036. Epub 2017 May 26.
Emergence of antibiotic resistance among pathogenic bacteria encourages us to search for new molecules as an alternative treatment. The aim of this study was to evaluate the antiquorum sensing (anti-QS) and antibiofilm potential of Salvadora persica L. methanolic extracts to prevent the infections due to Staphylococcus as an alternate to antibiotics. The methanolic extracts of S. persica L. fruit, leaves and stems was assessed for their activity in inhibiting QS-depedent phenomenon such as violacein pigment production in Chromobacterium violaceum, swarming motility of Pseudomonas aeruginosa PAO1 and biofilm formation in oral Staphylococcus strains on polymethylmetacrylate (PMMA). Methanolic fruit extract of S. persica L. showed a high degree of anti-biofilm formation on PMMA and on violacein inhibition with a percentage of reduction equal to 90% when MIC value (20 mg/ml) was used. 100 μg/ml of S. persica L. leaves exhibited inhibition in swarming motility of PAO1 at 29.17%. Because the methanolic extracts of S. persica L. demonstrated anti-QS and antibiofilm activity at very low concentrations, it could be further exploited for novel molecules to treat oral Staphylococcus infections.
病原菌中抗生素耐药性的出现促使我们寻找新的分子作为替代治疗方法。本研究的目的是评估佩氏骆驼蓬甲醇提取物的群体感应抑制（抗QS）和抗生物膜潜力，以预防葡萄球菌感染，作为抗生素的替代品。对佩氏骆驼蓬果实、叶子和茎的甲醇提取物抑制QS依赖性现象的活性进行了评估，这些现象包括紫色杆菌中紫色菌素色素的产生、铜绿假单胞菌PAO1的群游运动以及聚甲基丙烯酸甲酯（PMMA）上口腔葡萄球菌菌株的生物膜形成。佩氏骆驼蓬果实的甲醇提取物在PMMA上显示出高度的抗生物膜形成能力，在使用MIC值（20mg/ml）时对紫色菌素的抑制率达到90%。100μg/ml的佩氏骆驼蓬叶子对PAO1的群游运动的抑制率为29.17%。由于佩氏骆驼蓬的甲醇提取物在极低浓度下就表现出抗QS和抗生物膜活性，因此可以进一步开发新分子来治疗口腔葡萄球菌感染。
