荠蓝的抗老化感应和抗生物膜潜能。

Antiquorum sensing and antibiofilm potential of Capparis spinosa.

机构信息

Department of Biotechnology, Alagappa University, Karaikudi, Tamil Nadu, India.

出版信息

Arch Med Res. 2011 Nov;42(8):658-68. doi: 10.1016/j.arcmed.2011.12.002. Epub 2012 Jan 2.

DOI:10.1016/j.arcmed.2011.12.002

PMID:22222491

Abstract

BACKGROUND

Emergence of antibiotic resistance among bacterial pathogens often leads to the failure of existing antibiotics to treat bacterial infections; thus, there is a need to seek alternative treatment measures. The aim of this study was to evaluate the anti-quorum sensing (anti-QS) and antibiofilm potential of Capparis spinosa to prevent the onset of bacterial infections as an alternate to antibiotics.

METHODS

The methanolic extract of the dried fruits of C. spinosa was assessed for its activity in inhibiting QS-depedent phenomenon such as violacein pigment production in Chromobacterium violaceum, biosurfactant production in Pseudomonas aeruginosa PAO1, swimming and swarming motility, exopolysaccharide production (EPS) and biofilm formation in Escherichia coli, Proteus mirabilis, Serratia marcescens and PAO1.

RESULTS

Extract of C. spinosa showed a higher degree of anti-QS activity in a dose dependent manner without affecting the bacterial growth. At 2 mg/mL, this extract significantly (p ≤0.005) inhibited the biofilm formation to 79, 75, 73, 70% and EPS production to 58, 46, 66 and 67% in S. marcescens, PAO1, E. coli and P. mirabilis, respectively. It also exhibited inhibition in swimming and swarming motility of bacterial pathogens. The non-enzymatic nature of the anti-QS compound in C. spinosa was confirmed by proteinase K and heat treatment.

CONCLUSIONS

Because the methanolic extract of C. spinosa demonstrated anti-QS and antibiofilm activity at 0.5-2 mg/mL, it could be further exploited for novel molecules to treat the emerging infections of antibiotic resistant bacterial pathogens.

摘要

背景

细菌病原体对抗生素的耐药性的出现常常导致现有抗生素治疗细菌感染的失败；因此，需要寻求替代治疗措施。本研究旨在评估蓟的抗群体感应（anti-QS）和抗生物膜特性，以替代抗生素预防细菌感染的发生。

方法

评估了蓟的干果果实用甲醇提取物抑制依赖群体感应现象的活性，如在紫色色杆菌中产生紫色素、铜绿假单胞菌 PAO1 中产生生物表面活性剂、大肠杆菌、奇异变形杆菌、粘质沙雷氏菌和 PAO1 中的泳动和群集运动、胞外多糖（EPS）产生和生物膜形成。

结果

蓟提取物以剂量依赖的方式表现出更高程度的抗 QS 活性，而不影响细菌生长。在 2mg/mL 时，该提取物显著（p≤0.005）抑制生物膜形成至 79%、75%、73%和 70%，以及 EPS 产生至 58%、46%、66%和 67%，分别在粘质沙雷氏菌、PAO1、大肠杆菌和奇异变形杆菌中。它还表现出对细菌病原体泳动和群集运动的抑制。蓟中的非酶促抗 QS 化合物通过蛋白酶 K 和热处理得到证实。