溶组织内阿米巴抗原的代谢标记：一种28 kDa主要细胞内抗原的特性

Metabolic labeling of Entamoeba histolytica antigens: characterization of a 28-kDa major intracellular antigen.

作者信息

Bhattacharya A, Bhattacharya S, Sharma M P, Diamond L S

机构信息

Laboratory of Parasitic Diseases, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.

出版信息

Exp Parasitol. 1990 Apr;70(3):255-63. doi: 10.1016/0014-4894(90)90107-n.

DOI:10.1016/0014-4894(90)90107-n

PMID:2311709

Abstract

The in vivo incorporation of radiolabeled amino acids into antigens of Entamoeba histolytica, HM-1:IMSS, is reported. Immunoprecipitation with sera from patients with invasive amebiasis revealed a 28-kDa antigen present in whole cell lysates of E. histolytica. This antigen was of cytoplasmic origin, as indicated by cell fractionation and Triton X-114 detergent-phase separation. Immunoprecipitation, using sera from patients with invasive amebiasis and symptomless cyst passers, revealed the 28-kDa antigen as the major antigen recognized by the sera tested. Immunoprecipitation analysis using radiolabeled-released proteins instead of whole cell lysates showed a number of bands, including the 28-kDa antigen. The data suggest that the 28-kDa antigen is of cytoplasmic origin or is released from the cytoplasmic compartment.

摘要

据报道，放射性标记的氨基酸在体内掺入溶组织内阿米巴HM-1:IMSS抗原中。用侵袭性阿米巴病患者的血清进行免疫沉淀，发现溶组织内阿米巴全细胞裂解物中存在一种28 kDa的抗原。细胞分级分离和Triton X-114去污剂相分离表明，该抗原起源于细胞质。用侵袭性阿米巴病患者和无症状排包囊者的血清进行免疫沉淀，发现28 kDa抗原是所检测血清识别的主要抗原。使用放射性标记释放的蛋白质而非全细胞裂解物进行免疫沉淀分析，显示出多条条带，包括28 kDa抗原。数据表明，28 kDa抗原起源于细胞质或从细胞质区室释放。