Boatman D E, Bavister B D, Cruz E
Department of Veterinary Science, University of Wisconsin-Madison 53706.
J Androl. 1990 Jan-Feb;11(1):66-72.
Hamster epididymal spermatozoa became virtually immotile following washing and dilution in chemically defined medium (TLP-PVA). The sperm motility factors (penicillamine, hypotaurine, and epinephrine: PHE) were examined for their ability to reactivate immotile sperm. Sperm could be reactivated by addition of PHE at 1 h of incubation. Hypotaurine alone was capable of reactivating sperm motility, but epinephrine and penicillamine together were not. However, overall sperm motility and percentage of motile sperm during incubation were higher when PHE components were used in combination than when hypotaurine was used alone. Addition of hypotaurine to immotile sperm suspensions could be postponed for up to 6 h with subsequent recovery of sperm motility, although the degree of recovery of motility declined progressively with each hour that addition of hypotaurine was delayed. The rescuing effect of hypotaurine was due to an increase both in the percentage of motile sperm and in the quality (grade) of sperm motility. The data show that hypotaurine is required for expression of sperm motility in the hamster, and support the concept that the loss of hypotaurine from sperm following washing and dilution is responsible for the sperm-immobilizing effect of these procedures. Additionally, the data demonstrate that hamster sperm can remain viable for several hours after becoming immotile, and that many of the immotile sperm are capable of being reactivated.
仓鼠附睾精子在化学限定培养基（TLP-PVA）中洗涤和稀释后几乎失去运动能力。研究了精子运动因子（青霉胺、亚牛磺酸和肾上腺素：PHE）重新激活失活精子的能力。在孵育1小时时添加PHE可使精子重新激活。单独使用亚牛磺酸能够重新激活精子运动能力，但肾上腺素和青霉胺一起使用则不能。然而，与单独使用亚牛磺酸相比，联合使用PHE成分时，孵育期间精子的总体运动能力和活动精子百分比更高。向失活精子悬液中添加亚牛磺酸可推迟至6小时，随后精子运动能力恢复，尽管随着亚牛磺酸添加时间每延迟一小时，运动能力的恢复程度逐渐下降。亚牛磺酸的挽救作用是由于活动精子百分比和精子运动质量（等级）均增加。数据表明，仓鼠精子运动能力的表达需要亚牛磺酸，并支持这样的观点，即洗涤和稀释后精子中亚牛磺酸的丧失是这些操作导致精子固定作用的原因。此外，数据表明仓鼠精子在失去运动能力后仍可存活数小时，并且许多失活精子能够被重新激活。
