Barker S A, McDowell T, Charkhian B, Hsieh L C, Short C R
Louisiana State University, School of Veterinary Medicine, Department of Physiology, Pharmacology, and Toxicology, Baton Rouge 70803.
J Assoc Off Anal Chem. 1990 Jan-Feb;73(1):22-5.
Methodology for the analysis of 8 benzimidazoles as residues in bovine liver is reported. Spiked tissues were extracted by homogenization in saline and ammonium hydroxide and blending with diatomaceous earth. This matrix was packed into a column, and the benzimidazoles were eluted with ethyl acetate. After the sample was further purified, benzimidazoles were separated and quantitated by liquid chromatography with ultraviolet detection (290 nm). Liver tissue samples obtained from cattle which had undergone a drug depletion study of fenbendazole administered per os were analyzed using these methods. The results of these analyses and the application of this approach to multiresidue analysis of drugs in animal tissues are discussed.
本文报道了牛肝中8种苯并咪唑类药物残留的分析方法。加标组织经生理盐水和氢氧化铵匀浆，并与硅藻土混合后进行提取。将此基质填充到柱中，用乙酸乙酯洗脱苯并咪唑类药物。样品进一步纯化后，通过紫外检测（290nm）的液相色谱法分离并定量苯并咪唑类药物。使用这些方法分析了从经口服芬苯达唑药物消除研究的牛身上获取的肝组织样品。讨论了这些分析结果以及该方法在动物组织中药物多残留分析中的应用。
