Haga Makoto, Yamamoto Satoshi, Okamoto Hiroyuki, Hoshina Katsuyuki, Asakura Tetsuro, Watanabe Toshiaki
Division of Vascular Surgery, Department of Surgery, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
Department of Biotechnology, Tokyo University of Agriculture and Technology, Tokyo, Japan.
Ann Vasc Dis. 2017 Jun 25;10(2):132-138. doi: 10.3400/avd.oa.16-00118.
To evaluate in vivo patency rates of silk fibroin (SF) vascular grafts and resulting histological reactions in a canine model. To generate 3.5-mm inner diameter vessels, a combination of plaited silk fibers were wound with cocoon filaments and subsequently coated with an SF solution. The resulting SF grafts (n=35) were implanted into the carotid arteries of male beagles (age, 1-2 years; body weight: 9.0-10.5 kg). Expanded polytetrafluoroethylene (4-mm inner diameter, ePTFE) grafts (n=5) were used as controls. Graft patency was monitored via ultrasonography with histological changes analyzed via microscopic examination. Compared with animals that received the ePTFE grafts, animals that received SF grafts exhibited the same thickness of luminal layers and fibrin accumulation and collagen fiber replacement with endothelialization at 3 months post-implantation via histological examination. The patency rates of the SF and the ePTFE grafts at 6 months post-implantation were 7.8% and 0%, respectively. This canine model study demonstrated that SF grafts induce unique histological reactions but fail to achieve long-term patency.
评估丝素蛋白（SF）血管移植物在犬类模型中的体内通畅率以及由此产生的组织学反应。为制作内径3.5毫米的血管，将编织的丝纤维与茧丝缠绕在一起，随后用丝素蛋白溶液进行涂层处理。将所得的丝素蛋白移植物（n = 35）植入雄性比格犬（年龄1 - 2岁；体重9.0 - 10.5千克）的颈动脉中。使用内径4毫米的膨体聚四氟乙烯（ePTFE）移植物（n = 5）作为对照。通过超声检查监测移植物的通畅情况，并通过显微镜检查分析组织学变化。组织学检查显示，与接受ePTFE移植物的动物相比，接受丝素蛋白移植物的动物在植入后3个月时，管腔层厚度、纤维蛋白沉积以及胶原纤维替代和内皮化情况相同。植入后6个月时，丝素蛋白移植物和ePTFE移植物的通畅率分别为7.8%和0%。这项犬类模型研究表明，丝素蛋白移植物会引发独特的组织学反应，但无法实现长期通畅。
