Poutrel B, Mendolia C, Sutra L, Fournier J M
Station de Pathologie de la Reproduction, Institut National de la Recherche Agronomique, Nouzilly, France.
J Clin Microbiol. 1990 Feb;28(2):358-60. doi: 10.1128/jcm.28.2.358-360.1990.
Monoclonal antibodies to Staphylococcus aureus capsular polysaccharide types 5 and 8 were used in an enzyme-linked immunosorbent assay to serotype 74 and 42 coagulase-negative isolates from cow and goat milk, respectively. Eighteen (15.5%) isolates were typable: 13 Staphylococcus haemolyticus, 1 S. hyicus, 1 S. simulans, and 1 S. warneri from bovine origin and 2 S. lentus from caprine origin. Type 5 was predominant, accounting for about 89% of typable isolates. Reactivity with monoclonal antibodies varied considerably according to isolates. The significance and the potential importance of these findings are discussed.
利用针对金黄色葡萄球菌5型和8型荚膜多糖的单克隆抗体,通过酶联免疫吸附测定法分别对从牛奶和羊奶中分离出的74株和42株凝固酶阴性菌株进行血清分型。18株(15.5%)菌株可进行分型:13株溶血葡萄球菌、1株猪葡萄球菌、1株模仿葡萄球菌和1株沃氏葡萄球菌来自牛源,2株缓慢葡萄球菌来自羊源。5型为主型,约占可分型菌株的89%。不同菌株与单克隆抗体的反应性差异很大。本文讨论了这些发现的意义和潜在重要性。