型特异性单克隆抗体与金黄色葡萄球菌临床分离株及纯化荚膜多糖的反应性。
Reactivity of type-specific monoclonal antibodies with Staphylococcus aureus clinical isolates and purified capsular polysaccharide.
作者信息
Nelles M J, Niswander C A, Karakawa W W, Vann W F, Arbeit R D
出版信息
Infect Immun. 1985 Jul;49(1):14-8. doi: 10.1128/iai.49.1.14-18.1985.
Abstract
Staphylococcus aureus has been classified into at least eight different capsular types by using polyclonal rabbit antisera specific for their associated capsular polysaccharides. We produced and characterized monoclonal antibodies reactive with two serologically distinct capsular types, types 5 and 8, which account for more than 70% of all S. aureus bacteremias. These type-specific, monoclonal antibodies reacted with S. aureus clinical isolates possessing the homologous capsular type and exhibited no cross-reactivity against S. aureus clinical isolates possessing the heterologous capsular type, nontypeable S. aureus clinical isolates, Staphylococcus epidermidis clinical isolates, or a variety of gram-negative organisms. The anti-type 8 monoclonal antibodies also reacted with purified capsular polysaccharide derived from the prototype type 8 S. aureus strain.
摘要
通过使用针对相关荚膜多糖的多克隆兔抗血清，金黄色葡萄球菌已被分为至少八种不同的荚膜类型。我们制备并鉴定了与两种血清学上不同的荚膜类型（5型和8型）反应的单克隆抗体，这两种类型占所有金黄色葡萄球菌菌血症的70%以上。这些类型特异性的单克隆抗体与具有同源荚膜类型的金黄色葡萄球菌临床分离株反应，并且对具有异源荚膜类型的金黄色葡萄球菌临床分离株、不可分型的金黄色葡萄球菌临床分离株、表皮葡萄球菌临床分离株或多种革兰氏阴性菌没有交叉反应。抗8型单克隆抗体也与源自8型金黄色葡萄球菌原型菌株的纯化荚膜多糖反应。
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