Maulana Azad Medical College, New Delhi, India.
Arch Virol. 2013 Mar;158(3):571-81. doi: 10.1007/s00705-012-1522-5. Epub 2012 Nov 6.
Dengue is an important arboviral disease of tropical and subtropical regions, with significant morbidity and mortality. Dengue virus is antigenically classified into four serotypes, which are further classified into 4-5 genotypes based on their genetic diversity. Since genotypes vary in their virulence, their detection and analysis of spatial and temporal transition are essential. We utilized sequence information from the E-NS1 gene region for molecular and phylogenetic characterization of dengue viruses isolated from dengue patients between 2007 and 2009. All four serotypes and multiple genotypes were detected, with predominance and emergence of DENV-1 genotype V. Phylogenetic analysis revealed the emergence of DENV-1 genotype V from India for the first time, which has replaced the earlier circulating genotype III and genotype I. The circulation of multiple genotypes and genotype replacement is critical, since genotypes vary in their virulence, and this should be a point of concern for healthcare agencies.
登革热是一种发生在热带和亚热带地区的重要虫媒病毒性疾病，具有较高的发病率和死亡率。登革病毒根据抗原性分为四个血清型，根据其遗传多样性进一步分为 4-5 种基因型。由于各基因型的毒力不同，因此对其进行检测和分析时空转变非常重要。我们利用来自 2007 年至 2009 年登革热患者的 E-NS1 基因区域的序列信息，对登革热病毒进行了分子和系统发育特征分析。结果检测到了所有四个血清型和多个基因型，其中 DENV-1 基因型 V 占据优势并开始流行。系统发育分析显示，DENV-1 基因型 V 首次从印度出现，取代了先前流行的基因型 III 和基因型 I。多种基因型的流行和基因型更替非常关键，因为各基因型的毒力不同，这应该是医疗保健机构关注的一个重点。
