Division of Cancer Biology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, 466-8550, Japan.
Mol Cell Biochem. 2013 Feb;374(1-2):105-11. doi: 10.1007/s11010-012-1509-2. Epub 2012 Nov 6.
Dynamic remodeling of the actin cytoskeleton is crucial for biological processes such as cell migration and cell spreading. S100A10 is a member of the S100 protein family and is involved in intracellular trafficking and cell migration. In this study, we examined the role of S100A10 in actin cytoskeletal organization and cell spreading. Depletion of S100A10 induced disruption of stress fiber formation and delay in cell spreading. Rac1 activation during spreading was suppressed by S100A10 knockdown, and exogenous expression of active Rac1 restored the ability of cells to spread in the absence of S100A10. Our results demonstrate the crucial role of S100A10 in actin dynamics promoting cell spreading via Rac1 activation.
细胞骨架的动态重塑对于细胞迁移和细胞铺展等生物过程至关重要。S100A10 是 S100 蛋白家族的一员，参与细胞内运输和细胞迁移。在这项研究中，我们研究了 S100A10 在细胞骨架组织和细胞铺展中的作用。S100A10 的耗竭诱导应力纤维形成的破坏和细胞铺展的延迟。在铺展过程中 Rac1 的激活被 S100A10 的敲低抑制，并且外源性表达活性 Rac1 恢复了在没有 S100A10 的情况下细胞铺展的能力。我们的结果表明 S100A10 在通过 Rac1 激活促进细胞铺展的肌动蛋白动力学中的关键作用。
