伊朗溶组织内阿米巴分离株中tRNA基因座A-L的序列多样性
Sequence Diversity in tRNA Gene Locus A-L among Iranian Isolates of Entamoeba dispar.
作者信息
Nazemalhosseini-Mojarad E, Azimirad M, Nochi Z, Romani S, Tajbakhsh M, Rostami-Nejad M, Haghighi A, Zali Mr
机构信息
Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
出版信息
Iran J Parasitol. 2012;7(1):97-103.
BACKGROUND
A number of methods for detecting diversity in Entamoeba have been described over the years. In the present study the genetic polymorphism of noncoding locus A-L was analyzed using PCR and sequencing in order to clarify the genotypic differences among E. dispar isolates.
METHODS
A total of 28 E. dispar from patients with gastrointestinal symptoms were determined and the genomic DNA was extracted directly from stool. For genotype analysis; Locus A-L was amplified by PCR and PCR products were sequenced. The sequences obtained were edited manually and aligned using Gene Runner software.
RESULTS
With sequencing of PCR products a reliable genetic diversity in size, number and position of the repeat units were observed among the Iranian E. dispar isolates in locus A-L gene. Sequences showed variation in length from 448bp to 507bp and seven distinct types were identified.
CONCLUSION
The genetic diversity of loci like A-L shows them to be suitable for epidemiological studies such as the characterization of the routes of transmission of these parasites in Iran.
背景
多年来已经描述了多种检测溶组织内阿米巴多样性的方法。在本研究中，使用聚合酶链反应（PCR）和测序分析非编码位点A-L的基因多态性，以阐明不同种类的溶组织内阿米巴分离株之间的基因型差异。
方法
确定了总共28例有胃肠道症状患者的溶组织内阿米巴，直接从粪便中提取基因组DNA。进行基因型分析时，通过PCR扩增位点A-L，对PCR产物进行测序。手动编辑获得的序列，并使用Gene Runner软件进行比对。
结果
通过对PCR产物进行测序，在伊朗溶组织内阿米巴分离株的位点A-L基因中观察到重复单元的大小、数量和位置存在可靠的遗传多样性。序列长度在448bp至507bp之间变化，鉴定出七种不同类型。
结论
像A-L这样的位点的遗传多样性表明它们适用于流行病学研究，例如在伊朗对这些寄生虫传播途径的特征描述。
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