Hooshyar H, Rezaian M, Kazemi B, Jeddi-Tehrani M, Solaymani-Mohammadi S
Department of Medical Parasitology and Mycology, School of Public Health and Health Research, Tehran University of Medical Sciences, Tehran, Iran.
Parasitol Res. 2004 Sep;94(2):96-100. doi: 10.1007/s00436-004-1175-8.
The present study was carried out from August 1999 through February 2002 in order to determine the prevalence of Entamoeba histolytica and Entamoeba dispar in three different climatic regions of Iran by using a PCR-RFLP method. A total of 16,592 stool samples were randomly collected from different age-groups in central, northern, and southern Iran in both urban and rural areas. The samples were examined by direct and formalin-ether concentration methods. A total of 226 samples were positive for E. histolytica/E. dispar cysts. Of these, 101 isolates were cultured and maintained successfully in Robinson's medium and were identified by the PCR-RFLP method. The study showed that 92.1% of isolates were E. dispar and 7.9% were E. histolytica or mixed infections. The ratio of E. histolytica to E. dispar was higher in southern regions (tropical and subtropical) than in the other two regions. This study demonstrated that E. dispar is the predominant species found among "cyst passers" in Iran.
本研究于1999年8月至2002年2月开展，目的是通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法确定伊朗三个不同气候区域溶组织内阿米巴和迪斯帕内阿米巴的流行情况。从伊朗中部、北部和南部城乡不同年龄组中随机收集了总共16592份粪便样本。样本通过直接涂片法和福尔马林-乙醚浓缩法进行检查。共有226份样本的溶组织内阿米巴/迪斯帕内阿米巴包囊呈阳性。其中，101株分离株在罗宾逊培养基中成功培养并保存，通过PCR-RFLP方法进行鉴定。研究表明，92.1%的分离株为迪斯帕内阿米巴，7.9%为溶组织内阿米巴或混合感染。溶组织内阿米巴与迪斯帕内阿米巴的比例在南部地区（热带和亚热带）高于其他两个地区。本研究表明，迪斯帕内阿米巴是伊朗“排包囊者”中发现的主要种类。
