Feng Meng, Pandey Kishor, Yanagi Tetsuo, Wang Ting, Putaporntip Chaturong, Jongwutiwes Somchai, Cheng Xunjia, Sherchand Jeevan B, Pandey Basu Dev, Tachibana Hiroshi
Department of Infectious Diseases, Tokai University School of Medicine, Isehara, Japan.
Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Fudan University, Shanghai, China.
Parasitol Res. 2018 Aug;117(8):2467-2472. doi: 10.1007/s00436-018-5935-2. Epub 2018 May 28.
In Nepal, gastrointestinal infections due to parasites including Entamoeba species are common. The main aim of this study was to identify species of Entamoeba using genotypic analysis. The prevalence of Entamoeba infections was examined by PCR in fecal samples from 143 inhabitants living close to wild rhesus macaques in Kathmandu, Nepal. The numbers of positive cases were one (0.7%) for E. histolytica, eight (5.6%) for E. dispar, seven (4.9%) for E. coli, and two (1.4%) for E. chattoni (E. polecki ST2). No infections with E. nuttalli, E. moshkovskii, and E. polecki ST1 were found. In E. dispar, at least seven different genotypes were detected from the eight samples by sequence analysis of tRNA-linked short tandem repeats. Different genotypes were found even in a couple from the same family. This is the first report demonstrating that E. dispar with high genotypic diversity is prevalent, rather than E. histolytica, in Kathmandu, and that zoonotic transmission of E. chattoni from rhesus macaques might occur in the inhabitants.
在尼泊尔，包括内阿米巴属在内的寄生虫引起的胃肠道感染很常见。本研究的主要目的是通过基因分型分析来鉴定内阿米巴的种类。采用聚合酶链反应（PCR）检测了尼泊尔加德满都143名居住在野生恒河猴附近居民粪便样本中的内阿米巴感染情况。阳性病例数为：溶组织内阿米巴1例（0.7%），迪斯帕内阿米巴8例（5.6%），结肠内阿米巴7例（4.9%），查顿内阿米巴（波列基内阿米巴ST2）2例（1.4%）。未发现纳氏内阿米巴、莫斯科维茨内阿米巴和波列基内阿米巴ST1感染。在迪斯帕内阿米巴中，通过tRNA连接的短串联重复序列分析，从8个样本中至少检测到7种不同的基因型。甚至在来自同一家族的一对夫妇中也发现了不同的基因型。这是第一份报告，表明在加德满都流行的是具有高基因型多样性的迪斯帕内阿米巴，而非溶组织内阿米巴，并且恒河猴的查顿内阿米巴可能在居民中发生人畜共患病传播。
