Ali Ibne Karim M, Zaki Mehreen, Clark C Graham
Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, United Kingdom.
J Clin Microbiol. 2005 Dec;43(12):5842-7. doi: 10.1128/JCM.43.12.5842-5847.2005.
We have developed a reliable method for PCR-based genotyping of Entamoeba histolytica based on variation in the numbers of short tandem repeats that are linked to tRNA genes in this species. Species-specific primer pairs were designed that differentiate E. histolytica from E. dispar as well as that reveal intraspecies PCR product length polymorphisms. The primers were tested with samples from different parts of the world, and DNA was extracted from cultured cells as well as liver abscess pus and feces by various methods. We now have the tools necessary to investigate a possible link between parasite genotype and the outcome of infection with Entamoeba histolytica, as well as other aspects of the organism's epidemiology.
我们基于溶组织内阿米巴（Entamoeba histolytica）中与tRNA基因相连的短串联重复序列数量的变化，开发了一种可靠的基于PCR的基因分型方法。设计了物种特异性引物对，可区分溶组织内阿米巴和迪斯帕内阿米巴（E. dispar），并揭示种内PCR产物长度多态性。用来自世界各地的样本对引物进行了测试，并通过各种方法从培养细胞、肝脓肿脓液和粪便中提取DNA。我们现在拥有了必要的工具，可用于研究寄生虫基因型与溶组织内阿米巴感染结果之间可能存在的联系，以及该生物体流行病学的其他方面。
