Department of General Surgery, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
J Dig Dis. 2013 Jan;14(1):45-50. doi: 10.1111/j.1751-2980.2012.00645.x.
To determine the distribution of macrophages (MΦ) in both xanthogranulomatous cholecystitis (XGC) and gallbladder carcinoma (GBC) and to analyze the association between XGC and GBC.
From January 2009 to June 2011, 110 patients with gallbladder diseases, including 35 with GBC, 45 with XGC and 30 with chronic cholecystitis (CC), were enrolled. Immunohistochemistry stain and real-time polymerase chain reaction using oncogenes (BCL-2, c-Myc) and anti-oncogene genes (p53, p21) were performed, serum expressions of tumor marker (CA19-9, CA724 and CA242) were also conducted. MΦ were used to determine their potential role in the carcinogenesis of GBC.
BCL-2 and c-Myc expressions gradually increased among CC, XGC and GBC (P = 0.032 and P = 0.020, respectively); while p53 and p21 were similar in the three groups (P = 0.167 and P = 0.122, respectively). Serum BCL-2 and c-Myc were significantly correlated with their tissue levels; in terms of serum tumor markers, which gradually increased among CC, XGC and GBC, however, CA242 and CA724 were both negative in XGC but positive in GBC. Furthermore, gradually increasing MΦ counts were observed among CC, XGC and GBC groups; c-Myc and CA724 were independent predictors for the differentiation of XGC and GBC.
XGC is an uncommon inflammatory condition distinct from CC and may be associated with the precancerous nature of GBC for its upregulated oncogenes and MΦ biology. c-Myc and CA724 were independent predictors for the differentiation of XGC and GBC.
确定巨噬细胞（MΦ）在黄色肉芽肿性胆囊炎（XGC）和胆囊癌（GBC）中的分布，并分析 XGC 和 GBC 之间的关系。
2009 年 1 月至 2011 年 6 月，纳入 110 例胆囊疾病患者，包括 35 例 GBC、45 例 XGC 和 30 例慢性胆囊炎（CC）患者。采用免疫组织化学染色和实时聚合酶链反应检测癌基因（BCL-2、c-Myc）和抑癌基因（p53、p21），检测肿瘤标志物（CA19-9、CA724 和 CA242）的血清表达。MΦ 用于确定其在 GBC 发生中的潜在作用。
BCL-2 和 c-Myc 的表达在 CC、XGC 和 GBC 中逐渐增加（P=0.032 和 P=0.020）；而 p53 和 p21 在三组中相似（P=0.167 和 P=0.122）。血清 BCL-2 和 c-Myc 与其组织水平显著相关；就血清肿瘤标志物而言，其在 CC、XGC 和 GBC 中逐渐增加，但 XGC 中 CA242 和 CA724 均为阴性，而 GBC 中则为阳性。此外，在 CC、XGC 和 GBC 组中观察到逐渐增加的 MΦ 计数；c-Myc 和 CA724 是 XGC 和 GBC 分化的独立预测因子。
XGC 是一种与 CC 不同的罕见炎症性疾病，可能与 GBC 的癌前性质有关，因为其上调的癌基因和 MΦ 生物学。c-Myc 和 CA724 是 XGC 和 GBC 分化的独立预测因子。
