Centers for Disease Control and Prevention, Rickettsial Zoonoses Branch, Atlanta, Georgia, USA.
J Clin Microbiol. 2013 Jan;51(1):314-7. doi: 10.1128/JCM.01723-12. Epub 2012 Nov 7.
Two novel real-time PCR assays were developed for the detection of Rickettsia spp. One assay detects all tested Rickettsia spp.; the other is specific for Rickettsia rickettsii. Evaluation using DNA from human blood and tissue samples showed both assays to be more sensitive than nested PCR assays currently in use at the CDC.
两种新型实时 PCR 检测法被开发用于检测立克次体属。一种检测法可检测所有检测到的立克次体属;另一种则是针对立氏立克次体的。使用来自人类血液和组织样本的 DNA 进行评估显示,这两种检测法均比疾控中心目前使用的巢式 PCR 检测法更灵敏。