Lim Young Chang, Lee Seong-Ho, Song Mee Hyun, Yamaguchi Kiyoshi, Yoon Joo-Heon, Choi Eun Chang, Baek Seung Joon
Department of Pathobiology, College of Veterinary Medicine, University of Tennessee, 2407 River Drive, Knoxville, TN 37996-4542, United States.
Eur J Cancer. 2006 Dec;42(18):3260-6. doi: 10.1016/j.ejca.2006.07.014. Epub 2006 Oct 12.
In recent studies, green tea components have been shown to induce cell growth arrest and apoptosis in head and neck squamous cell carcinoma (HNSCC) cells. In this report, we have investigated the effects of epicatechin gallate (ECG), one of the catechins in green tea, on anti-cancer activity in vitro. We found that cyclin D1 was highly expressed in HNSCC cells, and ECG suppressed 90% of cyclin D1 expression in SCC7 cells. We have also evaluated the effect of ECG on cell growth and apoptosis, showing that ECG (50 microM) exhibited a significant inhibition (50%) on the growth of SCC7 cells via G1 cell cycle arrest. ECG suppressed cyclin D1 in SCC7 cells in a dose- and time-dependent manner, and the suppression of the beta-catenin pathway by ECG is one of the mechanism to facilitate ECG-induced cell growth arrest. These results suggest that ECG has a potential usage as a chemopreventive agent in HNSCC.
在最近的研究中，绿茶成分已被证明可诱导头颈部鳞状细胞癌（HNSCC）细胞的细胞生长停滞和凋亡。在本报告中，我们研究了绿茶中的儿茶素之一表没食子儿茶素没食子酸酯（ECG）对体外抗癌活性的影响。我们发现细胞周期蛋白D1在HNSCC细胞中高表达，并且ECG抑制了SCC7细胞中90%的细胞周期蛋白D1表达。我们还评估了ECG对细胞生长和凋亡的影响，结果表明ECG（50微摩尔）通过G1期细胞周期停滞对SCC7细胞的生长表现出显著抑制（50%）。ECG以剂量和时间依赖性方式抑制SCC7细胞中的细胞周期蛋白D1，并且ECG对β-连环蛋白途径的抑制是促进ECG诱导的细胞生长停滞的机制之一。这些结果表明ECG在HNSCC中具有作为化学预防剂的潜在用途。
