Ricieri Brito João Artur, Gomes Carolina Cavaliéri, Santos Pimenta Flávio Juliano Garcia, Barbosa Alvimar Afonso, Prado Marco Antônio Máximo, Prado Vânia Ferreira, Gomez Marcus Vinícius, Gomez Ricardo Santiago
Department of Oral Surgery and Pathology, School of Dentistry.
Exp Ther Med. 2010 Jan;1(1):217-221. doi: 10.3892/etm_00000035. Epub 2010 Jan 1.
MicroRNAs (miRNAs) mir15a and let7a are important regulators of bcl-2, ras and c-myc proteins. Considering that these miRNAs are commonly altered in many human cancers and that these proteins are reported to be altered in oral squamous cell carcinoma (OSCC), we investigated them in a set of OSCC cases. The miRNAs as well as the proteins were evaluated in the tumor and blood of 20 patients by real-time quantitative PCR and immunohistochemistry, respectively. The expression of mir15a and bcl-2 proteins in the tumors was not associated with each other or with tumor staging. On the other hand, we found reduced expression of this miRNA in the blood of patients with an advanced stage of OSCC and with lymph node metastasis. The expression of let7a in the tumor and blood was not associated with tumor size, lymph node metastasis, tumor staging and immunoexpression of ras and c-myc proteins. In conclusion, the present study shows that reduced expression of mir15a is associated with OSCC staging.
微小RNA(miRNA)mir15a和let7a是bcl-2、ras和c-myc蛋白的重要调节因子。鉴于这些miRNA在许多人类癌症中普遍发生改变,且据报道这些蛋白在口腔鳞状细胞癌(OSCC)中也发生改变,我们在一组OSCC病例中对它们进行了研究。分别通过实时定量PCR和免疫组织化学方法对20例患者的肿瘤组织和血液中的miRNA以及蛋白进行了评估。肿瘤组织中mir15a和bcl-2蛋白的表达彼此之间以及与肿瘤分期均无关联。另一方面,我们发现处于OSCC晚期且伴有淋巴结转移的患者血液中该miRNA表达降低。肿瘤组织和血液中let7a的表达与肿瘤大小、淋巴结转移、肿瘤分期以及ras和c-myc蛋白的免疫表达均无关联。总之,本研究表明mir15a表达降低与OSCC分期相关。