Department of Life and Environmental Sciences, University of Cagliari, via Ospedale 72, 09124 Cagliari, Italy.
J Agric Food Chem. 2012 Nov 28;60(47):11675-80. doi: 10.1021/jf3038874. Epub 2012 Nov 19.
In the present study, evaluated was the paralysis activity of whole Italian and Algerian Melia azedarach, commonly known as chinaberry, fruits and parts (seeds, wood, and kernels) against Meloidogyne incognita second stage juveniles (J(2)). The paralysis activity was evaluated in vitro after 1 h and 1 day immersion periods of nematodes in test solutions. Phenolic constituent components of the extracts were identified and quantified by high-performance liquid chromatography-mass spectrometry, while confirmation was performed by high-performance liquid chromatography-diode array. The water extract of the Italian M. azedarach fruit pulp (IPWE) showed significant nematicidal activity (EC(50/48h) = 955 μg/mL) and among its active ingredient components were p-coumaric acid and p-hydroxybenzoic acid (EC(50/48h) = 840 and 871 μg/mL, respectively). This is the first report of the nematicidal activity of M. azedarach pulp water extract and phenolic acids against the root knot nematode M. incognita.
在本研究中，评估了全意大利和阿尔及利亚楝树（俗称苦楝）果实和部分（种子、木材和果仁）对南方根结线虫第二阶段幼虫（J(2)）的麻痹活性。将线虫在测试溶液中浸泡 1 小时和 1 天后，在体外评估麻痹活性。通过高效液相色谱-质谱法鉴定和定量提取物中的酚类成分，并通过高效液相色谱-二极管阵列进行确认。意大利楝树果实果肉的水提取物（IPWE）表现出显著的杀线虫活性（EC(50/48h) = 955 μg/mL），其活性成分包括对香豆酸和对羟基苯甲酸（EC(50/48h) = 840 和 871 μg/mL）。这是首次报道楝树果肉水提取物和酚酸对南方根结线虫的杀线虫活性。
