Department of Virology 1, National Institute of Infectious Diseases, Toyama, Shinjuku-ku, Tokyo 162-8640, Japan.
Arch Virol. 2013 Mar;158(3):639-50. doi: 10.1007/s00705-012-1532-3. Epub 2012 Nov 9.
Progressive multifocal leukoencephalopathy (PML) is caused by JC polyomavirus (JCV) infection in the brain. JCV isolates from PML patients have variable mutations in the non-coding control region (NCCR) of the genome. This study was conducted to examine sequential changes in NCCR patterns of JCV isolates obtained from the cerebrospinal fluid (CSF) of PML patients. CSF specimens were collected from PML patients at different time points, the NCCR sequences were determined, and their compositions were assessed by computer-based analysis. In patients showing a marked increase in JCV load, the most frequent NCCR sequences in the follow-up specimens were different from those in the initial samples. In contrast, the dominant NCCRs in the CSF remained unaltered during the follow-up of individuals in whom the viral load decreased after therapeutic intervention. These data demonstrate that the majority of JCV variants emerge with the progression of PML and that these changes are suppressed when the viral load is decreased.
进行性多灶性白质脑病（PML）是由脑部的 JC 多瘤病毒（JCV）感染引起的。来自 PML 患者的 JCV 分离株在基因组的非编码控制区（NCCR）中有可变突变。本研究旨在检查从 PML 患者的脑脊液（CSF）中获得的 JCV 分离株的 NCCR 模式的连续变化。从 PML 患者在不同时间点采集 CSF 标本，确定 NCCR 序列，并通过基于计算机的分析评估其组成。在 JCV 载量明显增加的患者中，随访标本中最常见的 NCCR 序列与初始样本不同。相比之下，在治疗干预后病毒载量下降的个体的随访中，CSF 中的主要 NCCR 保持不变。这些数据表明，大多数 JCV 变体随着 PML 的进展而出现，并且当病毒载量降低时，这些变化会受到抑制。
