Department of Food Science and Nutrition, University of Ulsan, Ulsan 680-749, South Korea.
J Agric Food Chem. 2012 Dec 5;60(48):11935-41. doi: 10.1021/jf303548k. Epub 2012 Nov 26.
In this study, we investigated effects of pine nut oil (PNO) on high-fat-diet (HFD)-induced obesity and metabolic dysfunction in skeletal muscle and brown adipose tissue (BAT). Male C57BL/6 mice were fed a HFD with 15% energy from lard and 30% energy from either soybean oil (SBO-HFD) or PNO (PNO-HFD) for 12 weeks. The PNO-HFD resulted in less weight gain and intramuscular lipid accumulation than the SBO-HFD and was accompanied by upregulation of transcripts and proteins related to oxidative metabolism and phosphorylation of AMP-activated protein kinase (AMPK), as well as molecules selectively expressed in type I and type IIa muscle fibers. In addition, uncoupling protein-1 was upregulated in BAT. These beneficial metabolic effects were partly associated with the dual ligand activity of pinolenic acid, which is abundant in PNO, for peroxisome proliferator-activated receptors α and δ. Our findings suggest that PNO may have potential as a dietary supplement for counteracting obesity and metabolic dysregulation.
在这项研究中，我们研究了松子油（PNO）对高脂肪饮食（HFD）诱导的骨骼肌和棕色脂肪组织（BAT）肥胖和代谢功能障碍的影响。雄性 C57BL/6 小鼠喂食含 15%猪油和 30%大豆油（SBO-HFD）或 PNO（PNO-HFD）的 HFD，共 12 周。与 SBO-HFD 相比，PNO-HFD 导致体重增加和肌肉内脂质积累减少，同时与氧化代谢和 AMP 激活蛋白激酶（AMPK）磷酸化相关的转录物和蛋白质以及选择性表达在 I 型和 IIa 型肌纤维中的分子上调。此外，BAT 中的解偶联蛋白 1 上调。这些有益的代谢效应部分与 PNO 中丰富的 pinolenic 酸对过氧化物酶体增殖物激活受体 α 和 δ 的双重配体活性有关。我们的研究结果表明，PNO 可能作为一种膳食补充剂，用于对抗肥胖和代谢失调。
