University of Split Faculty of Chemistry and Technology, Teslina 10, 21000 Split, Croatia.
Food Chem. 2011 Jul 15;127(2):427-33. doi: 10.1016/j.foodchem.2011.01.013. Epub 2011 Jan 9.
This study aimed to research the influence of phenophase on the phenolic profile (phenolic acids, flavonoids and stilbenes) and related antioxidant properties of sage, one of the most characteristic plants of Dalmatian karst. The total phenol and flavonoid contents in sage leaves were determined spectrophotometrically, while the principal phenolics were determined using HPLC-RP-DAD. Antioxidant properties of this well known medicinal plant were determined as free radical scavenging activity (DPPH), ferric reducing/antioxidant power (FRAP) and by Briggs-Rauscher oscillating reaction (BR). The results strongly indicate that Dalmatian sage leaves are rich source of valuable phenolics, mainly phenolic acids, with extremely good antioxidant properties. The presence of resveratrol or its derivatives was confirmed in all extracts. The best results for total phenols and flavonoids, as well as the best antioxidant properties were obtained for May sage, while the highest amounts of catechin monomers and compounds from group of stilbenes were found in February extract.
本研究旨在研究物候期对鼠尾草（一种最典型的达尔马提亚喀斯特植物）酚类物质（酚酸、类黄酮和芪类化合物）组成和相关抗氧化特性的影响。采用分光光度法测定鼠尾草叶片中的总酚和总黄酮含量，同时采用高效液相色谱-反相二极管阵列检测法（HPLC-RP-DAD）测定主要酚类物质。采用 DPPH 自由基清除活性、铁还原/抗氧化能力（FRAP）和 Briggs-Rauscher 振荡反应（BR）测定这种著名药用植物的抗氧化特性。结果表明，达尔马提亚鼠尾草叶片是一种有价值的酚类物质（主要为酚酸）的丰富来源，具有极好的抗氧化特性。所有提取物中均证实存在白藜芦醇或其衍生物。5 月鼠尾草的总酚和总黄酮含量以及抗氧化性能最好，2 月提取物中儿茶素单体和芪类化合物的含量最高。
