Department of Biomedical Science, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
BMC Complement Altern Med. 2014 Jun 27;14:205. doi: 10.1186/1472-6882-14-205.
Immunomodulators are substances that modify immune system response to a threat. Immunomodulators modulate and potentiate the immune system, keeping it highly prepared for any threat. The immunomodulatory effect of the traditional medicine Tinospora crispa is investigated in this work.
T. crispa ethanol extract was fractionated by using different solvents. The ethanol extract and effective isolated fraction were used to investigate the potential immunomodulatory effect of different T. crispa doses ranging from 25 μg/mL to 1000 μg/mL on RAW 246.7 cells by detecting intracellular INF-γ, IL-6, and IL-8 expressions. The antioxidant activity of T. crispa was evaluated through FRAP and DPPH. The total phenolic and total flavonoid contents were also quantified.
Results show that T. crispa extract has higher antioxidant potential than ascorbic acid. The FRAP value of T. crispa extract is 11011.11 ± 1145.42 μmol Fe(+2)/g, and its DPPH inhibition percentage is 55.79 ± 7.9, with 22 μg/mL IC50. The results also reveal that the total phenolic content of T. crispa extract is 213.16- ± 1.31 mg GAE/g dry stem weight, and the total flavonoid content is 62.07- ± 39.76 mg QE/g dry stem weight. T. crispa crude extract and its isolated fraction significantly stimulate RAW264.7 cell viability (P ≤ 0.05) and intracellular INF-γ, IL-6, and IL-8 expressions. The results of LC-MS show that four of the active compounds detected in the T. crispa isolated fraction are cordioside, quercetin, eicosenoic acid (paullinic acid), and boldine.
The results of this study obviously indicate that T. crispa has immunomodulatory effects through the stimulation of INF-γ, IL-6, and IL-8 expressions. LC-MS phytochemical analysis showed that the T. crispa fraction has cordioside, quercetin, eicosenoic acid (paullinic acid), and boldine, which may be responsible for the immunostimulator effect of T. crispa.
免疫调节剂是一类能够调节机体免疫系统应答的物质。免疫调节剂可以调节和增强免疫系统，使其对任何威胁都保持高度警惕。本研究旨在探讨传统药物三叶鬼针草的免疫调节作用。
采用不同溶剂对三叶鬼针草乙醇提取物进行分级。使用不同浓度（25μg/mL 至 1000μg/mL）的三叶鬼针草乙醇提取物及其有效分离部位，检测细胞内 IFN-γ、IL-6 和 IL-8 的表达，以评估不同剂量的三叶鬼针草对 RAW 246.7 细胞的潜在免疫调节作用。采用 FRAP 和 DPPH 法评估三叶鬼针草的抗氧化活性，并定量测定总酚和总黄酮含量。
结果表明，三叶鬼针草提取物具有比抗坏血酸更高的抗氧化潜力。三叶鬼针草提取物的 FRAP 值为 11011.11±1145.42μmol Fe(+2)/g，DPPH 抑制率为 55.79±7.9%，IC50 为 22μg/mL。结果还表明，三叶鬼针草提取物的总酚含量为 213.16-±1.31mg GAE/g 干茎重，总黄酮含量为 62.07-±39.76mg QE/g 干茎重。三叶鬼针草粗提取物及其分离部位可显著刺激 RAW264.7 细胞活力（P≤0.05）和细胞内 IFN-γ、IL-6 和 IL-8 的表达。LC-MS 结果表明，在三叶鬼针草分离部位检测到的 4 种活性化合物为毛蕊异黄酮苷、槲皮素、二十碳烯酸（巴柳酸）和育亨宾。
本研究结果明显表明，三叶鬼针草通过刺激 IFN-γ、IL-6 和 IL-8 的表达发挥免疫调节作用。LC-MS 植物化学分析表明，三叶鬼针草部位含有毛蕊异黄酮苷、槲皮素、二十碳烯酸（巴柳酸）和育亨宾，这可能是三叶鬼针草免疫刺激作用的原因。
