Oishi I, Maeda A, Kurimura T, Kimura M
Biken J. 1979 Jun;22(2):61-9.
There have been three recent outbreaks of acute gastroenteritis in primary schools in Osaka prefecture caused by rotavirus: one in a school (TA) in April, 1974 and two in two other schools (TE and K) in May 1975. The morbidity from the disease was 2.4--15.8% for all age groups in the schools, and 20.1--34.1% for a certain age group. The disease lasted for 4 (K) to 14 days (TE). The first cases in schools TA and TE were followed by successive cases. The main clinical symptoms were higher frequencies of diarrhea (68--71.4%) and fever (78%) than in winter vomiting disease. There was no difference in the incidences of the disease in boys and girls. From observation on the disease in individual families, the incubation period seemed to be 3 days. Using paired sera and Neonatal Calf Diarrhea Virus (NCDV) as antigen, positive seroconversion was demonstrated by the complement fixation test and marked seroconversion by the indirect fluorescent antibody technique. With these techniques, specific antibody to NCDV was detectable in the sera from an early stage of illness. Rotavirus was found on electron microscopic examination of some fecal specimens of patients in TA, but not in those of patients in TE or K, although adenovirus was isolated from one patient. A serological survey of healthy children aged 0 to 12 showed that rotavirus is a common virus in Osaka.
1974年4月在一所学校（TA）发生一起，1975年5月在另外两所学校（TE和K）各发生一起。各学校所有年龄组的发病率为2.4%至15.8%，某一年龄组的发病率为20.1%至34.1%。病程持续4天（K校）至14天（TE校）。TA校和TE校的首例病例之后出现了连续发病情况。主要临床症状为腹泻（68%至71.4%）和发热（78%）的发生率高于冬季呕吐病。该病在男孩和女孩中的发病率没有差异。通过对个别家庭中疾病的观察，潜伏期似乎为3天。使用双份血清和新生小牛腹泻病毒（NCDV）作为抗原，通过补体结合试验证明有阳性血清转化，通过间接荧光抗体技术证明有明显血清转化。利用这些技术，在疾病早期的血清中可检测到针对NCDV的特异性抗体。在对TA校部分患者粪便标本进行电子显微镜检查时发现了轮状病毒，但在TE校或K校患者的标本中未发现，不过从一名患者身上分离出了腺病毒。对0至12岁健康儿童的血清学调查表明，轮状病毒在大阪是一种常见病毒。
