Oishi I, Yamazaki K, Minekawa Y, Nishimura H, Kitaura T
Biken J. 1985 Jun;28(1-2):9-19.
Studies were made by electron microscopy (EM) on the viruses associated with diarrhea of outpatients at a pediatric clinic in Osaka Prefecture during the three year period from 1980 through 1982. The viruses detected by EM by negative staining with phosphotungstic acid (PTA) were classified morphologically into 6 groups: rotavirus, adenovirus and four kinds of small spherical viruses, calicivirus, astrovirus, picornavirus/parvovirus (P/P)-like agent and Osaka-agent. Osaka-agent seems to be a newly identified small virus. It is 35-40 nm in diameter with a fringe of spike-like structures on its surface. Viruses were detected in 181 of the 395 cases of diarrhea (45.8%). Rotavirus was detected in 122 (30.9%) of the total cases and in 67.4% of the virus-positive cases, while other viruses were detected in 15% of the total cases; adenovirus in 23 (6%) and small agents in 36 (9%). Rotavirus infection showed a distinctive seasonal variation, being mainly restricted to cooler months, but infections with other viruses did not show any seasonal variation. The age distribution of patients suggested that infants of 0 to 2 years old are very susceptible to all viruses. Attempts to cultivate these viruses in vitro were successful with only two isolates of adenovirus type 5.
在1980年至1982年的三年期间，利用电子显微镜（EM）对大阪府一家儿科诊所门诊腹泻患者所感染的病毒进行了研究。通过磷钨酸（PTA）负染法在电子显微镜下检测到的病毒在形态上分为6组：轮状病毒、腺病毒以及四种小球形病毒，即杯状病毒、星状病毒、微小核糖核酸病毒/细小病毒（P/P）样病原体和大阪病原体。大阪病原体似乎是一种新发现的小型病毒。其直径为35 - 40纳米，表面有一圈穗状结构。在395例腹泻病例中有181例（45.8%）检测到病毒。在所有病例中有122例（30.9%）检测到轮状病毒，在病毒阳性病例中占67.4%，而其他病毒在所有病例中占15%；腺病毒23例（6%），小型病原体36例（9%）。轮状病毒感染呈现出明显的季节性变化，主要集中在较凉爽的月份，但其他病毒感染未表现出任何季节性变化。患者的年龄分布表明，0至2岁的婴儿对所有病毒都非常易感。仅对两株5型腺病毒分离株进行体外培养成功。
