WCU Biomodulation Major, Department of Agricultural Biotechnology, Seoul National University, Seoul, Korea.
PLoS One. 2012;7(11):e49204. doi: 10.1371/journal.pone.0049204. Epub 2012 Nov 7.
S-adenosylhomocysteine hydrolase-like protein 1 (AHCYL1), also known as IP(3) receptor-binding protein released with IP(3) (IRBIT), regulates IP(3)-induced Ca(2+) release into the cytoplasm of cells. AHCYL1 is a critical regulator of early developmental stages in zebrafish, but little is known about the function of AHCYL1 or hormonal regulation of expression of the AHCYL1 gene in avian species. Therefore, we investigated differential expression profiles of the AHCYL1 gene in various adult organs and in oviducts from estrogen-treated chickens. Chicken AHCYL1 encodes for a protein of 540 amino acids that is highly conserved and has considerable homology to mammalian AHCYL1 proteins (>94% identity). AHCYL1 mRNA was expressed abundantly in various organs of chickens. Further, the synthetic estrogen agonist induced AHCYL1 mRNA and protein predominantly in luminal and glandular epithelial cells of the chick oviduct. In addition, estrogen activated AHCYL1 through the ERK1/2 signal transduction cascade and that activated expression of AHCYL1 regulated genes affecting oviduct development in chicks as well as calcium release in epithelial cells of the oviduct. Also, microRNAs, miR-124a, miR-1669, miR-1710 and miR-1782 influenced AHCYL1 expression in vitro via its 3'-UTR which suggests that post-transcriptional events are involved in the regulation of AHCYL1 expression in the chick oviduct. In conclusion, these results indicate that AHCYL1 is a novel estrogen-stimulated gene expressed in epithelial cells of the chicken oviduct that likely affects growth, development and calcium metabolism of the mature oviduct of hens via an estrogen-mediated ERK1/2 MAPK cell signaling pathway.
S-腺苷同型半胱氨酸水解酶样蛋白 1（AHCYL1），也称为与 IP（3）结合后释放的 IP（3）受体结合蛋白（IRBIT），调节细胞内 IP（3）诱导的细胞质 Ca（2+）释放。AHCYL1 是斑马鱼早期发育阶段的关键调节因子，但对于 AHCYL1 的功能或 AHCYL1 基因在禽类中的激素调节知之甚少。因此，我们研究了 AHCYL1 基因在各种成年器官和雌激素处理鸡的输卵管中的差异表达谱。鸡 AHCYL1 编码一个 540 个氨基酸的蛋白质，高度保守，与哺乳动物 AHCYL1 蛋白具有高度同源性（>94%的同一性）。AHCYL1 mRNA 在鸡的各种器官中大量表达。此外，合成雌激素激动剂主要诱导鸡输卵管腔上皮细胞和腺上皮细胞中 AHCYL1 mRNA 和蛋白的表达。此外，雌激素通过 ERK1/2 信号转导级联激活 AHCYL1，激活的 AHCYL1 表达调节基因影响小鸡输卵管的发育以及输卵管上皮细胞中的钙释放。此外，miR-124a、miR-1669、miR-1710 和 miR-1782 通过其 3'-UTR 影响体外 AHCYL1 的表达，这表明转录后事件参与鸡输卵管中 AHCYL1 表达的调节。总之，这些结果表明 AHCYL1 是一种新型的雌激素刺激基因，在鸡输卵管的上皮细胞中表达，可能通过雌激素介导的 ERK1/2 MAPK 细胞信号通路影响母鸡成熟输卵管的生长、发育和钙代谢。
