O'Day D H
Can J Microbiol. 1979 Dec;25(12):1416-26. doi: 10.1139/m79-221.
A microcinematographic analysis of the behaviour and movements of cells and cell masses in mated cultures (NC4 X VI2) of Dictyostelium discoideum indicates that a chemotactic process directs cell aggregation during macrocyst development. Zygote giant cells form before aggregation begins and act as the aggregation centres. Young multicellular macrocyst stages are sources of cyclic AMP, and amoebae from macrocyst cultures orient chemotactically to cyclic AMP. The data, coupled with other characteristics such as pulsatile streaming, suggest that the aggregation process leading to macrycyst development is the same as that occurring during fruit construction. Other aspects of sexual development are also discussed. Based upon these data, we propose a model for the sequence of events leading to macrocyst development in D. discoideum.
对盘基网柄菌交配培养物（NC4×VI2）中细胞及细胞团行为和运动的显微电影分析表明，趋化过程在大囊泡发育期间指导细胞聚集。合子巨细胞在聚集开始前形成并充当聚集中心。年轻的多细胞大囊泡阶段是环磷酸腺苷（cAMP）的来源，来自大囊泡培养物的变形虫会以趋化方式定向至cAMP。这些数据，再加上诸如脉动流动等其他特征，表明导致大囊泡发育的聚集过程与子实体构建期间发生的过程相同。还讨论了有性发育的其他方面。基于这些数据，我们提出了一个盘基网柄菌中大囊泡发育事件序列的模型。
